园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 221-223.

• 研究报告 • 上一篇    下一篇



  1. ( 山东省果树研究所,泰安 271000; 西北农林科技大学园艺学院,杨凌 712100)
  • 收稿日期:2003-06-30 修回日期:2003-08-12 出版日期:2004-04-25 发布日期:2004-04-25

Studies on System of Plant Regeneration from Leaf Explant of Cherry Rootstocks

Huang Wenjiang ;Liu Qingzhong ;Fan Shenghua ;Zhao Hongjun; Ma Fengwang

  1. ( Shandong Institute of Pomology,Tai'an 271000,China; College of Horticulture,Northwestern Science and Technology University of Agriculture and Forestry,Yangling 712100,China)
  • Received:2003-06-30 Revised:2003-08-12 Online:2004-04-25 Published:2004-04-25

摘要: 以甜樱桃矮化砧木‘Gisela 5’和‘Gisela 6’的组培苗为试材,研究建立叶片的离体再生体系。结果表明,Gisela 6的再生能力高于Gisela 5;叶片正面接触培养基比背面接触培养基更有利于分化再生。用新梢顶端1~3节新展开的叶片制备外植体,分别接种在WPM+BA 7 mg/L+IBA 0.3 mg/L和WPM+BA 5 mg/L+IBA 0.5 mg/L的培养基上,黑暗处理15 d,Gisela 5的最高分化频率为75.1%,Gisela 6为100%。再生芽苗在三角瓶中生根容易,移人大田后生长正常。

关键词: 樱桃, 砧木, 矮化, 离体叶片, 再生体系, 组织培养

Abstract: An efficient and simple method for shoots regeneration from in vitro leaves of ‘Gisela 5’ and ‘Gisela 6’ (Pruns cerasus×P.canescens)was developed.Factors influencing in vitro leaf regeneration were studied.The ability to regenerate shoot from in vitro leaves of‘Gisela 6’ was much greater than that of ‘Gisela 5’:Optimal results were obtained by culturing leaves adaxial side down on WPM medium containing BA 5 mg/L+IBA 0.5 mg/L and BA 7 mg/L+IBA 0.3 mg/L respectively in the dark for 15 days.Shoot regeneration rate of ‘Gisela 6’ reached up to 10o% and that of ‘Gisela 5’ was 75.1% .The regeneration shoot was easy to rooting and to transplant to the field.

Key words: Cherry, Rootstock, Dwarf, In vitro leaf, Regeneration, Tissue culture