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园艺学报 ›› 2025, Vol. 52 ›› Issue (5): 1271-1284.doi: 10.16420/j.issn.0513-353x.2024-0913

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

芸薹属种间杂交聚合创制高抗根肿病新材料

蒋顺顺1,2, 初梦玉1, 杨槿曦1, 高梦杰1, 方治国2, 吴磊1, 任雪松1, 李勤菲1, 宋洪元1, 司军1,*()   

  1. 1 西南大学园艺园林学院,重庆市蔬菜学重点实验室,长江上游农业生物安全与绿色生产教育部重点实验室,重庆 400715,
    2 襄阳市农业科学院,湖北襄阳 441057
  • 收稿日期:2025-03-18 修回日期:2025-04-11 出版日期:2025-05-23 发布日期:2025-05-21
  • 通讯作者:
    *E-mail:
  • 基金资助:
    川渝联合实施重点研发项目(cstc2021jscx-cylh0001); 四川省科技计划资助项目(2024YFHZ0106); 国家级大学生创新创业训练计划资助项目(202410635091)

Brassica Interspecies Hybridization and Polymerization Creates New Materials for High Resistance to Clubroot

JIANG Shunshun1,2, CHU Mengyu1, YANG Jinxi1, GAO Mengjie1, FANG Zhiguo2, WU Lei1, REN Xuesong1, LI Qinfei1, SONG Hongyuan1, SI Jun1,*()   

  1. 1 Chongqing Key Laboratory of Vegetable Science,Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River,Ministry of Education,College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
    2 Xiangyang Academy of Agricultural Sciences,Xiangyang,Hubei 441057,China
  • Received:2025-03-18 Revised:2025-04-11 Published:2025-05-23 Online:2025-05-21

摘要:

对甘蓝9LQ(CRb)与大白菜8574(CRd)、芜菁ECD4(PbBa8.1)进行远缘杂交结合胚挽救技术,对杂种后代进行分子标记辅助选择,共筛选出7株包含两个抗性位点的材料(两抗材料),形态学和花粉活力鉴定表明,杂交后代均与亲本不同,9LQ × ECD4不能产生花粉,8574 × ECD4花粉育性正常。用两抗材料与Z3(CRbkato)杂交,共获得27株杂种后代,通过分子标记辅助选择,筛选出4株包含3个抗性位点的材料(三抗材料),形态学和花粉活力鉴定表明,杂交后代形态特征各异,3R-9、3R-10、3R-19的花粉育性均为100%,3R-26表现部分不育。将三抗材料与9LQ进行杂交,共获得8株杂交后代,用B0902、Yau389、A08-300、OA 4对引物进行分子标记辅助选择,成功筛选到1株包含4个抗性位点的材料(四抗材料)4R-6,形态学鉴定表明,四抗材料生长健壮,叶片开始有结球性状。鉴定结果表明,四抗材料对来自多个地区的根肿菌生理小种表现出优良的抗性。

关键词: 芸薹属, 远缘杂交, 根肿病, 基因聚合, 分子标记辅助选择

Abstract:

Cabbage 9LQ(CRb)was carried out with Chinese cabbage 8574(CRd)and turnip ECD4(PbBa8.1)by distantly hybridized combined embryo rescue technology,and the hybrid progeny was selected by molecular marker-assisted selection,and a total of 7 strains containing two resistance sites(two antibody materials)were screened,and the morphology and pollen vigor identification showed that the hybrid progeny were different from the parents,9LQ × ECD4 could not produce pollen,and the pollen fertility of 8574 × ECD4 was normal. A total of 27 hybrid progeny were obtained by hybridization with Z3(CRbkato)with amphibiotic materials,and 4 strains containing 3 resistance sites(tri-antibody materials)were screened out by molecular marker-assisted selection,and the morphological and pollen vigor identification showed that the morphological characteristics of the hybrid progeny were different,the pollen fertility of 3R-9,3R-10 and 3R-19 was 100%,and the expression of 3R-26 was partially sterile. A total of 8 hybrid progeny were obtained by hybridizing the triplicate material with 9LQ,and four pairs of primers B0902,Yau389,A08-300 and OA were used for molecular marker-assisted selection,and one strain containing four resistance sites(4R-6)was successfully screened,and morphological identification showed that the quadruple antibody material grew robustly and the leaves began to have ball-setting traits. The identification of resistance to clubroot showed that the four resistant materials showed excellent resistance to the physiological races of Plasmodiophora brassicae from many regions.

Key words: Brassica, distant hybridization, clubroot disease, gene polymerization, molecular marker-assisted selection