茄子全基因组InDel变异特征及分子标记开发和应用

doi:10.16420/j.issn.0513-353x.2022-1176

园艺学报 ›› 2024, Vol. 51 ›› Issue (3): 520-532.doi: 10.16420/j.issn.0513-353x.2022-1176

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

茄子全基因组InDel变异特征及分子标记开发和应用

尤园园¹^,²^,^*, 王帅²^,^*, 方莹莹¹, 齐诚¹, 李淑培¹, 张映¹, 陈钰辉¹, 刘伟³, 刘富中¹^,^**(), 舒金帅¹^,^**()

¹ 中国农业科学院蔬菜花卉研究所，蔬菜生物育种全国重点实验室，北京 100081
² 河北科技师范学院园艺科技学院，河北秦皇岛 066004
³ 中国农业科学院寿光蔬菜研发中心，山东寿光 262704

收稿日期:2023-07-05 修回日期:2023-08-23 出版日期:2024-03-25 发布日期:2024-03-22
通讯作者:
**E-mail：shujinshuai@caas.cn，
liufuzhong@caas.cn
作者简介:
* 共同第一作者
基金资助:
中央级公益性科研院所基本科研业务费专项(IVF-BRF2022003); 中央级公益性科研院所基本科研业务费专项(IVF-BRF2021006); 中国农业科学院创新工程项目(CAAS-ASTIP-IVFCAAS); 重庆市人民政府与中国农业科学院战略合作资助项目; 中蔬生物科技（寿光）有限公司企业研发项目(ZSSW-2023007); 中蔬生物科技（寿光）有限公司企业研发项目(ZSSW-2023008); 农业农村部园艺作物生物学与种质创制重点实验室项目

Variation Characteristics of Insertion-Deletion（InDel）and Molecular Markers Development and Application in Eggplant Based on Whole Genome Re-sequencing Data

YOU Yuanyuan¹^,², WANG Shuai², FANG Yingying¹, QI Cheng¹, LI Shupei¹, ZHANG Ying¹, CHEN Yuhui¹, LIU Wei³, LIU Fuzhong¹^,^**(), SHU Jinshuai¹^,^**()

¹ State Key Laboratory of Vegetable Biobreeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China
² College of Horticultural Science and Technology，Hebei Normal University of Science and Technology，Qinhuangdao，Hebei 066004，China
³ Shouguang Vegetable Research and Development Center，Chinese Academy of Agricultural Sciences，Shouguang，Shandong 262704，China

Received:2023-07-05 Revised:2023-08-23 Published:2024-03-25 Online:2024-03-22

摘要/Abstract

摘要：

选取4份圆茄（Solanum melongena L.）高代自交系进行全基因组重测序，鉴定出145 407个InDel，12号染色体InDel数最少（7 752），1号最多（19 738）。筛选出7 959个长度大于10 bp的InDel位点，挑选均匀分布在基因组上的144个设计InDel引物并进行验证。利用26份茄子材料对144对InDel引物进行筛选，63对表现出稳定的多态性，比例为43.75%；主要等位基因频率为0.4038 ∼ 0.9808，平均为0.7659；基因多样性指数（H_s）为0.0377 ~ 0.6501，平均为0.3217；多态性信息含量（PIC）为0.0370 ~ 0.5750，平均为0.2663，42个标记多态性信息含量大于0.200，多态性较好。当遗传距离为0.235时，将26份不同类型的茄子材料划分为2个类群。利用多态性信息含量高的前10个InDel标记构建了26份茄子材料DNA指纹图谱。另外，选择在亲本间具有多态性的InDel标记用于4个杂交组合的F₁代真实性鉴定，鉴定结果表明3个为真实杂交种，与田间鉴定结果吻合。

关键词: 茄子, InDel标记, 遗传多样性, 指纹图谱, F₁代真实性鉴定

Abstract:

Four high generation inbred lines of eggplant（Solanum melongena L.）were selected for whole genome re-sequencing，and 145 407 InDels were identified. The number of InDel on chromosome 12 was the least（7 752）and on chromosome 1 was the most（19 738）. There were 7 959 InDel with a length greater than 10 bp，and 144 InDels loci uniformly distributed on the genome were selected to be designed as InDel primers and verified. A total of 144 InDel primers were screened using 26 eggplant samples，63 primers showed stable polymorphism，and the polymorphism ratio was 43.75%. The main allele frequencies ranged from 0.4038 to 0.9808，with an average of 0.7659. Genetic diversity index（H_s）ranged from 0.0377 to 0.6501，with an average of 0.3217. The polymorphism information content（PIC）ranged from 0.0370 to 0.5750，with an average of 0.2663，and the PIC of 43 markers was greater than 0.200，indicating good polymorphism. When the genetic distance was 0.235，26 different types of eggplant materials were divided into two groups. The DNA fingerprints of 26 eggplant materials were constructed using the first ten InDel markers with high polymorphism information content. In addition，InDel markers with polymorphism among parents were selected for F₁ generation authenticity identification of four hybrid combinations，and three real hybrids were identified. The results from purity test of four hybrid combinations from InDel markers matched well with those from field experiments.

Key words: eggplant, Insertion-Deletion（InDel）marker, genetic diversity, fingerprint, F₁ identification

尤园园, 王帅, 方莹莹, 齐诚, 李淑培, 张映, 陈钰辉, 刘伟, 刘富中, 舒金帅. 茄子全基因组InDel变异特征及分子标记开发和应用[J]. 园艺学报, 2024, 51(3): 520-532.

YOU Yuanyuan, WANG Shuai, FANG Yingying, QI Cheng, LI Shupei, ZHANG Ying, CHEN Yuhui, LIU Wei, LIU Fuzhong, SHU Jinshuai. Variation Characteristics of Insertion-Deletion（InDel）and Molecular Markers Development and Application in Eggplant Based on Whole Genome Re-sequencing Data[J]. Acta Horticulturae Sinica, 2024, 51(3): 520-532.

图/表 8

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类型 Type	编号 Code	果色 Fruit color	萼片颜色 Sepal color	果形 Fruit shape	来源 Origin
自交系 Inbred line	21CNW1	紫黑 Purple black	紫Purple	圆Round	中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers，Chinese Academy of
	21CNW2	紫黑 Purple black	紫Purple	圆Round	Agricultural Sciences
	21CNW13	紫Purple	紫Purple	长Long
	21CNW14	紫Purple	紫Purple	长Long
	21CNW32	绿Green	绿Green	椭圆Oval
	21CNW33	绿Green	绿Green	椭圆Oval
F₁	21CNL764	紫Purple	紫Purple	长棒Long bar
F₁	21CNL770	紫色条纹 Purple stripe	绿Green	椭圆Oval
	21CNL743	紫黑 Purple black	紫Purple	圆Round	河北惠优特种业有限公司 Hebei Huiyoute Seed Industry Co.，LTD
	21CNL775	绿Green	绿Green	椭圆Oval	济南天瑞种子销售有限公司Jinan Tianrui Seed Sales Co.，LTD
	21CJD164	紫黑 Purple black	绿Green	短棒Short bar	山东省济南市Jinan，Shandong
	21CJD168	紫Purple	紫Purple	长Long	北京井田农业科技有限公司 Beijing Jingtian Agricultural Technology Co.，LTD
	21CJD170	绿Green	绿Green	椭圆Oval	辽宁园艺种苗有限公司 Liaoning Horticultural Seedling Co.，LTD
	21CJD171	绿Green	绿Green	椭圆Oval
	21CJD173	绿Green	绿Green	椭圆Oval	辽宁省鞍山市Anshan，Liaoning
	21CJD174	绿Green	绿Green	椭圆Oval
	21CJD175	绿Green	绿Green	椭圆Oval
	21CJD177	绿Green	绿Green	椭圆Oval
	21CJD179	绿Green	绿Green	椭圆Oval
	21CJD183	紫黑 Purple black	绿Green	短棒Short bar
	21CJD185	紫黑 Purple black	绿Green	短棒Short bar
	21CJD194	紫Purple	紫Purple	长Long
	21CJD184	紫Purple	绿Green	长棒Long bar	山东省潍坊市Weifang，Shandong
	21CJD195	紫Purple	紫Purple	长Long	四川广汉龙盛种业有限公司 Sichuan Guanghan Longsheng Seed Industry Co.，LTD
	21CJD196	紫黑 Purple black	紫Purple	长Long	辽宁省沈阳市Shenyang，Liaoning
自交系 Inbred line	21CJD197	紫红 Purple red	紫绿Purple green	线Line	浙江省杭州市Hangzhou，Zhejiang

类型 Type	编号 Code	果色 Fruit color	萼片颜色 Sepal color	果形 Fruit shape	来源 Origin
自交系 Inbred line	21CNW1	紫黑 Purple black	紫Purple	圆Round	中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers，Chinese Academy of
	21CNW2	紫黑 Purple black	紫Purple	圆Round	Agricultural Sciences
	21CNW13	紫Purple	紫Purple	长Long
	21CNW14	紫Purple	紫Purple	长Long
	21CNW32	绿Green	绿Green	椭圆Oval
	21CNW33	绿Green	绿Green	椭圆Oval
F₁	21CNL764	紫Purple	紫Purple	长棒Long bar
F₁	21CNL770	紫色条纹 Purple stripe	绿Green	椭圆Oval
	21CNL743	紫黑 Purple black	紫Purple	圆Round	河北惠优特种业有限公司 Hebei Huiyoute Seed Industry Co.，LTD
	21CNL775	绿Green	绿Green	椭圆Oval	济南天瑞种子销售有限公司Jinan Tianrui Seed Sales Co.，LTD
	21CJD164	紫黑 Purple black	绿Green	短棒Short bar	山东省济南市Jinan，Shandong
	21CJD168	紫Purple	紫Purple	长Long	北京井田农业科技有限公司 Beijing Jingtian Agricultural Technology Co.，LTD
	21CJD170	绿Green	绿Green	椭圆Oval	辽宁园艺种苗有限公司 Liaoning Horticultural Seedling Co.，LTD
	21CJD171	绿Green	绿Green	椭圆Oval
	21CJD173	绿Green	绿Green	椭圆Oval	辽宁省鞍山市Anshan，Liaoning
	21CJD174	绿Green	绿Green	椭圆Oval
	21CJD175	绿Green	绿Green	椭圆Oval
	21CJD177	绿Green	绿Green	椭圆Oval
	21CJD179	绿Green	绿Green	椭圆Oval
	21CJD183	紫黑 Purple black	绿Green	短棒Short bar
	21CJD185	紫黑 Purple black	绿Green	短棒Short bar
	21CJD194	紫Purple	紫Purple	长Long
	21CJD184	紫Purple	绿Green	长棒Long bar	山东省潍坊市Weifang，Shandong
	21CJD195	紫Purple	紫Purple	长Long	四川广汉龙盛种业有限公司 Sichuan Guanghan Longsheng Seed Industry Co.，LTD
	21CJD196	紫黑 Purple black	紫Purple	长Long	辽宁省沈阳市Shenyang，Liaoning
自交系 Inbred line	21CJD197	紫红 Purple red	紫绿Purple green	线Line	浙江省杭州市Hangzhou，Zhejiang

染色体 Chromosome	插入 Insertion	缺失 Deletion	总计 Total	InDel/%
E01	9 366	10 372	19 738	13.57
E02	4 164	4 737	8 901	6.12
E03	5 236	5 858	11 094	7.63
E04	4 855	5 320	10 175	7.00
E05	4 505	5 042	9 547	6.57
E06	6 132	6 879	13 011	8.95
E07	7 837	8 912	16 749	11.52
E08	5 734	6 468	12 202	8.39
E09	5 538	6 129	11 667	8.02
E10	4 394	5 091	9 485	6.52
E11	7 162	7 831	14 993	10.31
E12	3 640	4 112	7 752	5.33
染色体支架 Scaffold	42	51	93	0.06
总计 Total	68 605	76 802	145 407	100.00

染色体 Chromosome	插入 Insertion	缺失 Deletion	总计 Total	InDel/%
E01	9 366	10 372	19 738	13.57
E02	4 164	4 737	8 901	6.12
E03	5 236	5 858	11 094	7.63
E04	4 855	5 320	10 175	7.00
E05	4 505	5 042	9 547	6.57
E06	6 132	6 879	13 011	8.95
E07	7 837	8 912	16 749	11.52
E08	5 734	6 468	12 202	8.39
E09	5 538	6 129	11 667	8.02
E10	4 394	5 091	9 485	6.52
E11	7 162	7 831	14 993	10.31
E12	3 640	4 112	7 752	5.33
染色体支架 Scaffold	42	51	93	0.06
总计 Total	68 605	76 802	145 407	100.00

染色体Chromo-some	引物名Primer name	物理位置Physical location	正向引物（5′-3′） Forward primer sequence	反向引物（5′-3′） Reverse primer sequence
E01	EP01-1	7 915 894	CCCCAAGTGACCATTGGATTAG	AGATAGCTAGTTATTATCAACTTGGTGATTATATT
	EP01-2	23 527 396	CTTTAGTCAGGCTCAGGCTTATTC	GGTGACACTCGTAAGAACGCCTG
	EP01-8	64 525 306	GATAATTATCCAGTCGTCCACTTCA	CCCTTTGGGAGCAACTGAAAAATAAAATG
	EP01-3	100 413 397	CAAAAGAGATAATATGTGATTTGTTGCAAAT	TGTTCAGATACATCAATAATGTGCAAGATAC
	EP01-10	82 891 881	CCCTAAAACAAATGATGCATGAGGA	GGAATTTGAATTTTGAATTTTGAATTCAAACCCTAA
	EP01-12	105 994 712	GTTAAGACTGATTATTCTTCCCAAAAGAA	CCTCTGTTTATGACTTGTTTTAGAATATAAATTTCA
E02	EP02-1	1 654 359	GTGGGGGAGCATTGATTTAGTTAGA	TCCTTAAAGAATTCTAACTATCTACTTGTTATCTT
	EP02-9	54 917 742	AGAGAACAGATATAGGCACCCC	TATGGCTGCATAAACTTTTACTTCTATTCAC
	EP02-10	60 002 669	GACAATTGATGGTCCACGTGCT	GGAGAAACCAGCGTCTATGAGAATTATTT
	EP02-11	68 280 945	GGAACTGGAAAACAAAATGTGCAAC	GATGATTGTGAAAAGTTTAAGAATTGCAAGTAC
	EP02-12	74 262 259	ATTAGGGGAAGCGCTCCCTC	TTCTCCCTTAATTAGATATCTCGGCTAG
E03	EP03-1	6 139 884	GGTGGGGAGATTAGGATTTCTTC	CGTTAGGGTTTAACTAAATTTAGTTTCGTAC
	EP03-2	84 066 039	CTAACACATGACACATACCATAAGGT	GTACCTTGAACAAATCATGATCTGTTACTTTTAAA
	EP03-7	46 352 738	GACTAGACATGGACATGTGTAATTTC	GTTCTAGAGTTTAAAAATGGCTAAGTATGAAAG
	EP03-8	56 559 091	ATGAGACCCCGATTATGGGTCA	GAAGATTGGAAAAGAAAAATAGAACGACAAG
	EP03-11	77 552 027	CACCGAATTAGTAATACCTGCATTG	GCAATTTGGACGAATTAATTGACTTACTTCTAAA
	EP03-12	88 470 166	TTGGACTAATAGTACCACCCAAGAA	CTCATTTGGGTGAAATACTTCCTATTGATAATTT
E04	EP04-1	5 445 165	CTAGTGCCTAAGAAGGACGGAAA	CTTGGCATAATTGTCGATGAAAATATGAATG
	EP04-9	41 157 634	GGGTGTGTAATGCCAGTGTAAAAC	CTTTAGGGTGTTGCAAGGTGTTGATTG
	EP04-10	53 164 819	GGTGGATGAAGGACATTTTTGTACTA	CAGTTTTTGCTAGGATATGTAACTTAGTTATTC
	EP04-11	75 729 039	CTTTTAATCAAATGTTATGAGTTTGCATGT	CATCTATCGTTCAATATCGTATTGAAATTTGAC
	EP04-12	78 538 303	GAGTGATTACTTTTCTTTTATCATGTGTTC	TGTTACACTCATTGTTGACCTAGTTTTCTTT
E05	EP05-4	577 619	GATGTGTAGGCCATGAGCCC	CATGGAAAATGGCCGAAATCGCTTG
	EP05-7	43 441 050	AACAAATGAAAGAATGTACATTCTTCCAAT	AAGCTTTAGTACTTTCTTTTCAAACATTCTTTAAG
	EP05-9	60 366 228	CTCTTGACATCTTAGTTGCAAAAGC	GACTCCTTCACTTTGCCGCTTCTTC
	EP05-10	68 771 642	CAACATACCCTTACATAACTTGGCA	GTCTAGTCTAGCTAATGTTGGTTACTATC
	EP05-3	80 781 008	CTTTCGATTGACTAACTCGAGTGAAA	CAGAATGTAATGAACAACAAATCTTAGTTTGC
E06	EP06-4	884 474	CTTATTATGCTTGGCTTCAGCTTATATT	ATTTGTCTCCATGCCAACAATATTGTTTTTC
	EP06-1	9 494 444	CGTGGATAATCCTAATGAGAATCTGT	CTAACAAAAGCGTTCCTAAATTATTTCATAGC
	EP06-2	54 653 085	GTCAAAAATAGAGTCCTGTCTGGC	CATTGTTCCTGCTTTTAAAAAGAGGCAAC
	EP06-11	84 360 559	AAGATGCTTAAAGGTGTAACAGTCTAT	AAAGGTTGATAAATATTTGACAGAACACCAC
E07	EP07-1	4 004 708	CTATTATACTGCCTTTTGGTTGTCATTTT	GGTTAGAATCCACAAATTTCAAATCTTGAATTC
	EP07-4	14 816 419	CCGTCAACCAGAACATGTCGTTA	CTGTAGGAAGGTACAATATCTACATGTG
	EP07-6	46 991 068	CCTAAGGTATGTGGGTTTCTTTCC	CATGCAATTTGAGTAAAAGGAGGTCTGG
	EP07-7	56 540 678	GAAAGTCCCGAATGATATCATTGTC	GAAAATGATAGACATAAAATACTCATATTTTGCCC
	EP07-8	65 335 992	GGTCCCCTCTCGTTTAATATTTAGA	AACCATAACAACCAAGAGTCAAGAACAC
	EP07-3	94 455 988	GACAATGAATAAACAGAGACAGGGC	CAATTATATGTTTTACATATGAAAATCAGGCACATT
E08	EP08-1	4 461 068	CAAAGACAGACCCTAAATTTTTAGAATCAA	GATTGCTTAACTTGTGCTCAATTTGCAG
	EP08-7	39 745 348	ACTCAGACAACTGATTAAGAAGTGAAT	GAATCATGTTCAGGGTGATGTTGTTTTATG
	EP08-9	53 352 594	CCAAGACTTCATCAAATCTCTTATTCAT	GGAACCTTGAAGCTTGAGATGAGTTC
	EP08-10	66 951 767	GTCTCTCCGTCAATTTAAGTCCTAG	GTCTTTGCTTCATCACTTCGGATCAAC
	EP08-12	86 112 573	GAACCATTTTCTTTTCTTGGTTGTGAC	CAAATTGTCAACATAGTGTAAAGAAATTAGATGAAA
E09	EP09-8	41 897 213	AGTTAAATATTTGGAGGAAGTGAAGAC	GTAAAAGGAAAGCAAATAGCCTTACATACC
	EP09-10	56 706 456	TACAAGTTGGGTTCACCCCATATTT	ACTTCATAACTTGTAATGCAGGGATAATAAC
	EP09-2	66 581 625	GATTGATTTGCAACTTTCAAATCGATG	CTCAATTCCTTCGTCAAATTAATTCCCTC
	EP09-3	84 540 296	TAAAGAGGGTCCCGATCAACTTTTT	AGTAATTTACCATTTTAATTAGGCACAAGGC
E10	EP10-4	640 499	GGGGAAACGACGGTAGGAGATT	CTCAGCGGGTGACTAACTAAACTTTTAC
	EP10-5	22 305 109	GTCGTGCGTAACACACTATATATATATATA	AAACTGACCCACCGTACCCAGC
	EP10-6	32 848 394	CATCTTGAGTACCTCCTTGATCG	CCTTATGTCTGGTACAAGGGTAAGG
	EP10-7	36 204 090	TGAAAAGAACTACCCTACCAGTGAA	CCATACAAATAATGACGCCAAATCTTTAATG
	EP10-8	41 868 664	GCAAACCATGAAGAACTAACAAGGT	CCCTTGAAAACATGCAAAAAGAAGAGAG
	EP10-9	56 305 390	ATTCGTGTTGTTTGCTCATTCATCC	GTCCAAATGGTATTCATAAAAGGTCTTCTTG
	EP10-10	63 994 673	TTGAATGCTTATTTGAGTGTTTTATGGATT	CAACTCACATTCTTGATCTTAGCTATTCAAG
	EP10-3	80 974 089	TCATGTTATATGATTATGGCACATTCC	GCCTCTATAGTTGATGTTTTAGAGTTAATTTAG
E11	EP11-5	30 941 193	TGCCATTATAGTAACTGCTATGTATTC	CACCCTCAACTTAGAACTGTTGCTTG
	EP11-6	44 604 118	GGTATGTACCTCAATCACCTTGTC	TTGAGATATCTAACCCTCAACATGGTAG
	EP11-8	62 505 321	CCCTTCGAAATCGAGCAAGCAA	TTACTGAATGATCGATTCGGCCGAG
	EP11-9	71 529 170	CACCATGTCAGAGAAGATGGACA	CACCTTCGCATTTAAGAGGATATCATTC
	EP11-3	98 055 442	TGAAATGGAATTTTCCCCACATTGG	CAGAGACCTTACATTTATCTCTTTATAAGAG
E12	EP12-4	12 541 476	TGTTTAAGTCTTTACATGATGAGATTTGAA	CATTATCTCACATTCAAGTCATATGAAAAATTTTAC
	EP12-5	18 519 761	TAGAACCCGTATTGGGGTGTAG	GAAAAATTCTACTTAAGTCCCTAACCTTCAAA
	EP12-11	64 229 774	GCCTTGAATTTGGAGAAGCTGGA	GTAATGTATGCCACTGCACTATCCAAAC
	EP12-3	70 590 237	AAACAAACAACAATAATATATCTAGTGAAATC	CCCTTGCAATAGCTTTTTGGAAAAAGAATC

茄子全基因组InDel变异特征及分子标记开发和应用

Variation Characteristics of Insertion-Deletion（InDel）and Molecular Markers Development and Application in Eggplant Based on Whole Genome Re-sequencing Data

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标记名称Marker name	主等位基因频率Major allele frquency	基因型数Genotype number	N_a 等位基因数Number of allele	H_s 基因多样性指数Gene diversity index	H_e 期望杂合度 Expected heterozygosity	PIC 多态性信息含量Polymorphic information content
EP01-1	0.5192	3	2	0.4993	0.1923	0.3746
EP01-2	0.5192	3	2	0.4993	0.1923	0.3746
EP01-3	0.5192	3	2	0.4993	0.1923	0.3746
EP01-8	0.7115	4	3	0.4297	0.2692	0.3648
EP01-10	0.8462	3	2	0.2604	0.0769	0.2265
EP01-12	0.7308	3	2	0.3935	0.1538	0.3161
EP02-1	0.5962	3	2	0.4815	0.1923	0.3656
EP02-9	0.5769	4	3	0.5178	0.4615	0.4179
EP02-10	0.9423	2	2	0.1087	0.1154	0.1028
EP02-11	0.6923	3	2	0.4260	0.0769	0.3353
EP02-12	0.6923	3	2	0.4260	0.3077	0.3353
EP03-1	0.6923	3	2	0.4260	0.1538	0.3353
EP03-2	0.5962	3	2	0.4815	0.1154	0.3656
EP03-7	0.7500	3	2	0.3750	0.1923	0.3047
EP03-8	0.8462	3	2	0.2604	0.1538	0.2265
EP03-11	0.6731	3	2	0.4401	0.1923	0.3432
EP03-12	0.8462	3	3	0.2692	0.2308	0.2480
EP04-1	0.9423	2	2	0.1087	0.1154	0.1028
EP04-9	0.9038	4	3	0.1783	0.0385	0.1704
EP04-10	0.7308	4	3	0.4231	0.0769	0.3768
EP04-11	0.6346	4	3	0.5288	0.1154	0.4716
EP04-12	0.9231	2	2	0.1420	0	0.1319
EP05-3	0.7115	3	2	0.4105	0.2692	0.3262
EP05-4	0.8846	3	2	0.2041	0.1538	0.1833
EP05-7	0.7692	3	2	0.3550	0.3077	0.2920
EP05-9	0.9038	4	3	0.1783	0.0385	0.1704
EP05-10	0.8846	3	2	0.2041	0.0769	0.1833
EP06-1	0.7308	3	3	0.4231	0.3846	0.3768
EP06-2	0.9615	2	2	0.0740	0	0.0712
EP06-4	0.6154	4	3	0.5000	0.0769	0.4078
EP06-11	0.7692	3	2	0.3550	0.0769	0.2920
EP07-1	0.8077	3	2	0.3107	0.1538	0.2624
EP07-3	0.5962	3	2	0.4815	0.1154	0.3656
EP07-4	0.5192	3	2	0.4993	0.8077	0.3746
EP07-6	0.9615	2	2	0.0740	0.0769	0.0712
EP07-7	0.8077	3	2	0.3107	0.3077	0.2624
EP07-8	0.9423	3	2	0.1087	0.0385	0.1028
EP08-1	0.5192	3	2	0.4993	0.6538	0.3746
EP08-7	0.9808	2	2	0.0377	0.0385	0.0370
EP08-9	0.9423	3	2	0.1087	0.0385	0.1028
EP08-10	0.7115	3	2	0.4105	0.3462	0.3262
EP08-12	0.8269	3	2	0.2862	0.1923	0.2453
EP09-2	0.6923	4	3	0.4467	0.3077	0.3756
EP09-3	0.7500	4	3	0.3913	0.1923	0.3391
EP09-8	0.9231	3	2	0.1420	0.0769	0.1319
EP09-10	0.8462	3	2	0.2604	0.1538	0.2265
EP10-3	0.4038	6	3	0.6501	0.2692	0.5750
EP10-4	0.5192	3	2	0.4993	0.1923	0.3746
EP10-5	0.7885	2	2	0.3336	0.4231	0.2779
EP10-6	0.8462	3	2	0.2604	0.2308	0.2265
EP10-7	0.9231	2	2	0.1420	0	0.1319
EP10-8	0.9808	2	2	0.0377	0.0385	0.0370
EP10-9	0.7500	4	3	0.3913	0.1923	0.3391
EP10-10	0.9231	2	2	0.1420	0	0.1319
EP11-3	0.5769	3	2	0.4882	0.2308	0.3690
EP11-5	0.8846	3	2	0.2041	0.1538	0.1833
EP11-6	0.9231	3	2	0.1420	0.0769	0.1319
EP11-8	0.9231	3	2	0.1420	0.0769	0.1319
EP11-9	0.9231	2	2	0.1420	0.1538	0.1319
EP12-3	0.7308	4	3	0.4112	0.1538	0.3526
EP12-4	0.9423	2	2	0.1087	0.1154	0.1028
EP12-5	0.6346	3	2	0.4638	0.3462	0.3562
EP12-11	0.6346	3	2	0.4638	0.6538	0.3562
平均值Mean	0.7659	3.03	2.22	0.3217	0.1874	0.2663

编号Code	分子编码Molecular ID	编号Code	分子编码Molecular ID
21CNW1	1111111113	21CJD171	1223131112
21CNW2	1112111132	21CJD173	1213131112
21CNW13	2111132112	21CJD174	3332111113
21CNW14	3212121131	21CJD175	1233221113
21CNW32	1211131312	21CJD177	1223111323
21CNW33	1223111112	21CJD179	1233311133
21CNL743	1111111313	21CJD183	2122312231
21CNL764	2312332113	21CJD184	2232112231
21CNL770	3323122213	21CJD185	2323113221
21CNL775	1233211132	21CJD194	2123232231
21CJD164	2123113213	21CJD195	3112222221
21CJD168	2122332113	21CJD196	2112112133
21CJD170	3323111112	21CJD197	2213112231

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茄子全基因组InDel变异特征及分子标记开发和应用

Variation Characteristics of Insertion-Deletion（InDel）and Molecular Markers Development and Application in Eggplant Based on Whole Genome Re-sequencing Data

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