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园艺学报 ›› 2021, Vol. 48 ›› Issue (1): 173-182.doi: 10.16420/j.issn.0513-353x.2020-0236

• 研究报告 • 上一篇    下一篇

芍药黑斑病病原菌鉴定及其对杀菌剂敏感性分析

陶航, 扎依娜·玛合巴提, 张烨, 孙李瞳, 黄沈鑫, 张子辉, 刘旺, 施宁雪, 陈孝仁**()   

  1. 扬州大学园艺与植物保护学院,江苏扬州 225009
  • 收稿日期:2020-09-22 修回日期:2020-12-04 出版日期:2021-01-25 发布日期:2021-01-29
  • 通讯作者: 陈孝仁 E-mail:xrchen@yzu.edu.cn
  • 基金资助:
    国家自然科学基金项目(31671971);国家自然科学基金项目(31871907);江苏省农业科技自主创新资金项目(CX203125);扬州大学优秀青年骨干教师培养对象项目;扬州大学高端人才支持计划项目

Identification,Growth Conditions and Fungicide Sensitivity of the Pathogen Causing Black Spot Disease on Peony Leaves

TAO Hang, ZAYINA Mahabat, ZHANG Ye, SUN Litong, HUANG Shenxin, ZHANG Zihui, LIU Wang, SHI Ningxue, CHEN Xiaoren**()   

  1. College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China
  • Received:2020-09-22 Revised:2020-12-04 Online:2021-01-25 Published:2021-01-29
  • Contact: CHEN Xiaoren E-mail:xrchen@yzu.edu.cn

摘要:

对在江苏省扬州市芍药(Paeonia lactiflora)叶片上发现的一种黑斑病,采集其病叶,分离和纯化病原物,根据柯赫氏法则明确其致病性。基于病原物形态特征和多基因(rDNA-ITS、endoPGOPA2-1Alt a 1)序列联合分析,鉴定该病原物为链格孢(Alternaria alternata)。该病原菌的最适生长条件是:温度为28 ℃,pH 7.0,最适碳源为可溶性淀粉,最适氮源为硝酸钾。室内毒力测定发现,在测试的4种杀菌剂中,嘧菌酯和吡唑醚菌酯乳油对病原菌离体生长的抑制作用较强,而戊唑醇和异菌脲对其生长的抑制作用较差。

关键词: 芍药, 黑斑病, 链格孢, 生长条件, 杀菌剂

Abstract:

A black spot disease was discovered on peony(Paeonia lactiflora)leaves in Yangzhou City,Jiangsu Province in 2017. To determine the causing agent,the fungus was isolated and purified from the diseased leaves,and was tested for its pathogenicity following Koch’s postulation. The fungus was identified as Alternaria alternata based on its morphological characteristics and the combined sequence analyses of rDNA-ITS,endoPG,OPA2-1 and Alt a 1. Analysis of its growth conditions showed that the optimal growth conditions of A. alternata on Potato Dextrose Agar(PDA)include the temperature at 28 ℃ and pH 7.0. On defined Czapek media,soluble starch and KNO3 are the optimal carbon source and nitrogen source,respectively. What’s more,among four fungicides tested in toxicity assays,both Azoxystrobin and Pyraclostrobin exhibited more stronger inhibition effect on the mycelial growth of the pathogen on PDA,compared with Tebuconazole and Iprodione.

Key words: peony, leaf black spot disease, Alternaria, growth conditions, fungicide

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