番茄SlPLATZ18调控低温胁迫应答和ABA信号响应的功能分析

doi:10.16420/j.issn.0513-353x.2025-0092

摘要/Abstract

摘要：

PLATZ转录因子在植物非生物胁迫响应过程中起着重要作用。以番茄‘Micro-Tom’为材料，克隆了PLATZ家族转录因子基因SlPLATZ18，其开放阅读框（ORF）长687 bp，编码228个氨基酸。序列比对和系统进化树分析表明，SlPLATZ18与参与逆胁迫响应的马铃薯StPLATZ9/11和棉花GhPLATZ1具有较高的序列相似性。通过遗传转化获得SlPLATZ18过表达株系（SlPLATZ18-OE）。低温胁迫下，与野生型（WT）相比，SlPLATZ18-OE植株活性氧（ROS）积累减少；超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）活性增强；脯氨酸（Pro）含量增加；丙二醛（MDA）含量和相对电导率降低；低温响应基因SlCBF1、SlCBF3、SlCOR47-like和SlRD29A表达显著上调。过表达SlPLATZ18植株的低温抗性显著增强。此外，脱落酸（ABA）处理下，SlPLATZ18-OE种子的萌发率和初生根长度均显著高于野生型，表明其ABA敏感性降低，促进ABA处理下初生根的伸长。因此推测SlPLATZ18正调控番茄的低温胁迫应答，并负调控ABA信号响应过程。

关键词: 番茄, SlPLATZ18, 低温, ABA

Abstract:

PLATZ transcription factors play crucial roles in plant response to abiotic stress. The open reading frame（ORF）of SlPLATZ18 was cloned from‘Micro-Tom’tomato. The length of ORF was 687 bp，encoding 228 amino acids. Sequence alignment and phylogenetic tree analysis revealed that SlPLATZ18 had higher homology with StPLATZ9/11 and GhPLATZ1. The SlPLATZ18-overexpressing tomato lines （SlPLATZ18-OE）were generated via genetic transformation. Under low temperature stress，compared to the wild type（WT），SlPLATZ18-OE plants exhibited reduced the accumulation of reactive oxygen species（ROS），enhanced the activities of superoxide dismutase（SOD），peroxidase（POD）and catalase（CAT），increased the content of proline（Pro），decreased the content of malondialdehyde（MDA）and the relative conductivity. Additionally，the expressions of the cold-responsive genes（SlCBF1，SlCBF3，SlCOR47-like，and SlRD29A）were significantly upregulated in SlPLATZ18-OE than those of WT. The cold tolerance of SlPLATZ18-OE plants was markedly improved. Furthermore，under ABA treatment，the seed germination rates and primary root lengths of SlPLATZ18-OE were significantly higher than those of WT. It was speculated that SlPLATZ18 positively regulated low temperature stress response and negatively regulated ABA signal response in tomato.

Key words: tomato, SlPLATZ18, low temperature, ABA

樊蓓, 任敏, 王延峰, 陈国梁, 党峰峰, 程国亭, 孙会茹. 番茄SlPLATZ18调控低温胁迫应答和ABA信号响应的功能分析[J]. 园艺学报, 2025, 52(12): 3229-3242.

FAN Bei, REN Min, WANG Yanfeng, CHEN Guoliang, DANG Fengfeng, CHENG Guoting, SUN Huiru. Function Analysis of SlPLATZ18 in Regulating Low Temperature Stress and ABA Signal Response in Tomato[J]. Acta Horticulturae Sinica, 2025, 52(12): 3229-3242.

图/表 8

表1 本研究中所用的引物

Table 1 Primers used in this study

用途 Purpose	引物名称 Primer name	引物序列（5′-3′） Primer sequence
qRT-PCR	SlPLATZ18-Q-F	TCCACCAAAGTTTCAGTCCGT
	SlPLATZ18-Q-R	CGGAATCCCCTTTCTTCGCTT
	SlCBF1-Q-F	GAGTCGGAAGAAGTTTCAGG
	SlCBF1-Q-R	TGTAGGCATCAGTTTCCAC
	SlCBF3-Q-F	TGCCGGGTTTACTTACGAAT
	SlCBF3-Q-R	TCAGCTTCCACATGATCTCC
	SlCOR47-like-Q-F	TCTAGTAGCTCCAGTGATG
	SlCOR47-like-Q-R	TCTCCTCTGTTTCCTCGT
	SlRD29A-Q-F	AAAAATGAAGACGTGCATAGGC
	SlRD29A-Q-R	GTTTTCCAACGGAACCAACATA
	SlEF1α-Q-F	GACAGGCGTTCAGGTAAGG
	SlEF1α-Q-R	CCAATGGAGGGTATTCAGC
基因克隆Gene clone	SlPLATZ 18-ORF-F	ATGGGAGCTGGAGGACCTG
	SlPLATZ 18-ORF-R	TTAATAACCTATAACTAGTCCTCCC
过表达载体构建	SlPLATZ18-OE-F	CACGGGGGACTCTAGAATGGGAGCTGGAGGACCTG
Overexpression vector	SlPLATZ18-OE-R	GACGGCCAGTGAGCTCTTAATAACCTATAACTAGTCCTCCC
转基因株系鉴定 Identification	35S	CTATCCTTCGCAAGACCCTTC
overexpression tomato line	18-OE-R	ATACCTGTGAAGTCTGTGAAAG

图1 SlPLATZ18和其他物种PLATZ的系统进化树

Fig. 1 Phylogenetic tree of SlPLATZ18 and other species PLATZ proteins

图2 不同物种PLATZ蛋白的多序列比对 Sl：番茄；St：马铃薯；Gh：棉花；Phe：毛竹；At：拟南芥；Gm：大豆；Pt：毛果杨

Fig. 2 Multiple sequence alignment of PLATZ proteins from different species Sl：Solanum lycopersicum；St：S. tuberosum；Gh：Gossypium hirsutum；Phe：Phyllostachys edulis；At：Arabidopsis thaliana；Gm：Glycine max；Pt：Populus trichocarpa

图3 SlPLATZ18-OE番茄株系的PCR验证（A）和SlPLATZ18的表达分析（B） t检验。**表示SlPLATZ18-OE株系与野生型（WT）间存在显著差异（P < 0.01）。下同

Fig. 3 PCR validation（A）and SlPLATZ18 expression（B）of overexpressed tomato lines t-test. ** indicates significant differences between SlPLATZ18-OE and WT（P < 0.01）. The same below

图4 低温处理下SlPLATZ18-OE和WT番茄植株表型（A）、DAB染色和NBT染色（B）

Fig. 4 Phenotype（A）and DAB and NBT staining（B）of SlPLATZ18-OE and WT tomato under low temperature treatment

图5 低温处理下SlPLATZ18-OE和WT番茄植株的生理指标检测

Fig. 5 Physiological indices of SlPLATZ18-OE and WT tomato plants under low temperature treatment

图6 低温处理下SlPLATZ18-OE和WT番茄植株中低温相关基因的表达

Fig. 6 Expressions of cold-related genes in SlPLATZ18-OE and WT tomato plants under low temperature treatment * P < 0.05；** P < 0.01

图7 ABA处理下SlPLATZ18-OE和WT番茄种子萌发情况

Fig. 7 Seed germination of SlPLATZ18-OE and WT tomato under ABA treatment

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