关键词: 哈密瓜, 黄瓜花叶病毒, 寄主反应, 序列分析

Abstract:

The samples of Hami melon with virus-infected symptoms were collected from naturally infected plants growing in sixteen of the major melon-growing locations in Xinjiang. RT-PCR was used to detect Cucumber mosaic virus（CMV）in the samples. Seventeen CMV isolates were obtained from 12 major melon-growing locations by repeated single-local-lesion transfers in Vicia faba. Host reaction and sequence analysis of coat protein genes of the 17 CMV isolates were performed in this study. The results showed that the 17 CMV isolates were identified as the members of CMV belonging to subgroupⅠB and exhibited low genetic diversity. Overall，the molecular clustering of CMV isolates from Xinjiang was not well correlated with their geographic origin. Except for Datura stramonium，there were no obvious correlations of host responses to inoculation with CMV isolates from different geographical locations. Host reaction on different hosts revealed some differences compared to the CMV isolates collected from other countries.

Key words: Hami melon, Cucumber mosaic virus, host reaction, sequence analysis