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园艺学报 ›› 2013, Vol. 40 ›› Issue (10): 1935-1942.

• 观赏植物 • 上一篇    下一篇

建兰AP1基因的克隆、表达及其与MADS-box转录因子相互作用的分析

吴菁华,吴少华*,杨 超,张志忠   

  1. (福建农林大学园艺学院,福州 350002)
  • 收稿日期:2013-06-26 出版日期:2013-10-25 发布日期:2013-10-25

Cloning and Expression Analysis on AP1 Homologous Gene from Cymbidium ensifolium and Interaction Analysis Between AP1 and MADS-box Transcription Factors

WU Jing-hua,WU Shao-hua*,YANG Chao,and ZHANG Zhi-zhong   

  1. (College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
  • Received:2013-06-26 Online:2013-10-25 Published:2013-10-25

摘要: 为研究APl/SQUAMADS-box基因在建兰花发育中作用,以‘四季大青’建兰花芽为材料,用RT-PCR和RACE技术获得AP1基因,命名为CeAP1。序列分析表明,CeAP1基因cDNA全长866 bp,其开放阅读框编码一个含241个氨基酸的蛋白质。通过蛋白序列比对发现CeAP1与兰科植物的AP1蛋白质同源性较高。荧光定量PCR分析表明,CeAP1基因在建兰花发育初期高水平表达,同时在营养器官中也表达。酵母双杂交试验分析显示,CeAP1能自身形成同源二聚体,同时也能与除CeAG1外的其他MADS-box发生相互作用。

关键词: 建兰, 花发育, AP1同源基因, 相互作用

Abstract: To investigate the function of AP1 genes on flower development in Cymbidium ensifoliumAP1 was cloned from‘Sijidaqing’flower buds and the full length of AP1 cDNA was amplified by RACE method,named CeAP1. CeAP1 is 866 bp in length with a 726 nucleotides ORF that putatively encodes a protein with 241 amino acids. Homology analysis showed that the CeAP1 was highly homologous to the AP1MADS-box genes of orchids. Quantitative real-time PCR analysis demonstrated that CeAP1 was expressed in the initial period of floral development and vegetative organs. The yeast two-hybrid analysis indicated that the CeAP1was not only able to form homodimer but also formed heterodimers with the other MADS-box transcription factors except for CeAG1.

Key words: Cymbidium ensifolium, flower development, AP1 homologous gene, interaction

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