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园艺学报 ›› 2012, Vol. 39 ›› Issue (2): 375-380.

• 研究报告 • 上一篇    下一篇

过量表达tMEK2基因的大岩桐植株对温度胁迫的反应

孙姝兰1,温秋香1,代建丽1,邢  惕2,王小菁1,*   

  1. 1华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州 510631;2卡尔顿大学生物系,渥太华 K1S5B6,加拿大
  • 收稿日期:2011-11-01 修回日期:2012-01-03 出版日期:2012-02-25 发布日期:2012-02-25
  • 通讯作者: 王小菁 E-mail:wangxj@scnu.edu.cn

Responses of Sinningia speciosa Overexpressing tMEK2 to Temperature Stresses

SUN Shu-lan1,WEN Qiu-xiang1,DAI Jian-li1,XING Tim2,and WANG Xiao-jing1,*   

  1. 1Guangdong Key Lab of Biotechnology for Plant Development,School of Life Sciences,South China Normal University,Guangzhou 510631,China;2Department of Biology,Carleton University,Ottawa K1S5B6,Canada
  • Received:2011-11-01 Revised:2012-01-03 Online:2012-02-25 Published:2012-02-25
  • Contact: WANG Xiao-jing E-mail:wangxj@scnu.edu.cn

摘要: 构建番茄中MAPKK基因tMEK2的过量表达载体并转化到大岩桐中,通过PCR和Southern blot鉴定出转基因株系并对其进行抗性分析。结果表明,转基因植株与对照相比叶片变小,叶片的叶绿素含量显著增加,在4 ℃低温和40 ℃高温胁迫之后的恢复能力明显比对照增强。

关键词: 大岩桐, tMEK2, 过量表达, 温度胁迫, 恢复

Abstract: We constructed a MAPKK gene from tomato,tMEK2 overexpressing construct to transform into Sinningia speciosa. Transgenic plants were identified by PCR and Southern blotting analyses,and their resistance to cold and high temperatures was analyzed. The results showed that the tMEK2 overexpressing plants had smaller leaves,increased contents of chlorophyll in leaves,and better recoveries of plant growth after treatments with cold and high temperatures,compared to control of wild type plants.

Key words: Sinningia speciosa, tMEK2, overexpression, temperature stress, recovery of growth

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