关键词: 湖北海棠, 二价融合表达载体, MhTGA2转录因子, 几丁质酶基因, 耐盐性

Abstract: The plant binary expression vector containing the divalent genes of MhTGA2 and Mhchitl isolated from Malus hupehensis and fused in a single open reading frame（ORF）by foot and mouth disease virus（FMDV）were constructed. The vector was then introduced into tobacco via Agrobacterium-mediated. The expression of NtSOD，NtAPX and NtPPO genes were detected and salinity tolerance was evaluated in the tobacco transformants. The results showed that we successfully constructed a plant binary expression vector with bivalent fusion and denoted it as MhT2AC. The MhTGA2 gene and the Mhchitl gene were co-expression in the transformants. Comparing with the controls，the expression of NtSOD，NtAPX and NtPPO genes were enhanced in transgenic lines. Moreover，the salinity tolerance of the T₁ transformants were significantly improved.

Key words: Malus hupehensis, MhT2AC, MhTGA2, Mhchitl, salinity tolerance