果实软化相关PG基因的进化分析和基因组定位

园艺学报 ›› 2011, Vol. 38 ›› Issue (09): 1791-1799.

果实软化相关PG基因的进化分析和基因组定位

魏潇，刘威生*，刘宁，章秋平，张玉萍，刘硕，刘有春

（辽宁省果树科学研究所，国家果树种质熊岳李杏圃，辽宁熊岳 115009）

收稿日期:2011-06-14 修回日期:2011-08-16 出版日期:2011-09-25 发布日期:2011-09-25
通讯作者: 刘威生

Phylogenetic Analysis and Genomic Localization of Polygalacturonase Genes Related to Fruit Softening

WEI Xiao，LIU Wei-sheng*，LIU Ning，ZHANG Qiu-ping，ZHANG Yu-ping，LIU Shuo，and LIU You-chun

（Liaoning Institute of Pomology，National Germplasm Repository for Plums and Apricots，Xiongyue，Liaoning 115009，China）

Received:2011-06-14 Revised:2011-08-16 Online:2011-09-25 Published:2011-09-25
Contact: LIU Wei-sheng

摘要/Abstract

摘要： 利用NCBI数据库和蔷薇科基因组数据库中的数据对48个果实PG基因进行系统进化分析，结果表明3个进化支中都包含与果实软化相关的PG基因，属于A支和属于B支的PG基因有明显的功能差异。氨基酸序列分析显示，3个进化支中的PG基因存在多个差异位点，A支和C支PG基因的N端比B支短60 ~ 70个氨基酸。PG基因结构（包括启动子、内含子和编码区）的变异对其功能差异有重要影响。对苹果和桃的PG基因进行了基因组精确定位，其附近存在果实软化相关性状标记。

关键词: 多聚半乳糖醛酸酶基因, 果实软化, 系统进化分析, 基因组定位

Abstract: Forty-eight PG genes were classified into three major clades by phylogenetic analysis and each clade contained PG genes that involved in fruit softening. PG genes belonged to clade A and clade B had functional divergence obviously. There were some different amino acid sites in amino acid sequence of PG genes from different clades. PG genes in clade A and clade C had a deletion of 60–70 amino acids in N terminal end compared with PG genes in clade B. Variation in the structure of PG genes（mainly including promoter regions，introns and encoding regions）had a significant impact on their functions. PG genes in apple and peach were accurately localized in genome respectively and around the genes there were some fruit softening markers.

Key words: polygalacturonase genes, fruit softening, phylogenetic analysis, genomic localization

中图分类号:

S 66

魏潇;刘威生;刘宁;章秋平;张玉萍;刘硕;刘有春. 果实软化相关PG基因的进化分析和基因组定位[J]. 园艺学报, 2011, 38(09): 1791-1799.

WEI Xiao;LIU Wei-sheng;LIU Ning;ZHANG Qiu-ping;ZHANG Yu-ping;LIU Shuo;and LIU You-chun. Phylogenetic Analysis and Genomic Localization of Polygalacturonase Genes Related to Fruit Softening[J]. ACTA HORTICULTURAE SINICA, 2011, 38(09): 1791-1799.

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