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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 193-196.

• 观赏植物 • 上一篇    下一篇

蝴蝶兰无性快繁规模化生产的研究

顾德峰1 ; 赵春莉1 ;宋彦君2 ; 谷素敏2 ; 张家旭2 ; 张红伟1   

  1. (1吉林农业大学园艺学院, 长春130118; 2 吉林国联农业生物技术发展股份有限公司, 长春130022)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Studies on Crucial Technology of Rapid Asexual Propagation and CommercialProduction of Phalaenopsis

GU De-feng1 ; ZHAO Chun-li1 ; SONG Yan-jun2 ; GU Su-min2 ; ZHANG Jia-xu2 ; ZHANG Hong-wei1   

  1. (1 College of Horticulture, Jilin Agricultural University, Changchun 130118, China; 2 Jilin International Union AgriculturalBiology Technique Development Ltd. , Changchun 130022, China )
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 对影响蝴蝶兰( Phalaenopsis) 无性快繁规模化生产中常见技术问题进行了研究。研究表明:
基本培养基的类型和继代时间直接影响蝴蝶兰类原球茎( Protocorm-like-body, PLB) 的增殖速率, 在改良
狩野和改良KC培养基中类原球茎的增殖速率明显高于MS培养基; 对培养215个月的类原球茎进行转接继
代增殖率最高; 采用两步成苗法培养幼苗, 可将幼苗的出瓶时间缩短到7~8个月; 对5个品种扩繁20代
的类原球茎的开花株进行了跟踪调查, 结果是各品种开花株群体性状稳定, 3个品种无变异现象发生, 2个
品种的类原球茎在18~20代时变异率低于万分之一。

关键词: 蝴蝶兰, 组织培养, 规模化生产

Abstract: The major technology which affecting rapid asexual propagation and commercial production of
Phalaenopsiswas discussed. Results showed that the type of culture medium and the time of subculture directly
affected the p ropagation rate of its p rotocorm. The propagation rates of protocorm in the modified Kyoto and KC
media were significantly higher than that in MS medium. The op timal subculture time was 2.5 months. The
maturity of seedlingswas accelerated by using two step s cultivation, and it only needed seven or eightmonths
in tube. According to investigation on the abloom p lant of p rotocorm of five varieties p ropagated for 20 genera
tions, the results showed that the colony characters of these blooming plants are stable and the rate of variation
of two protocormswas lower than 1 /10 000 in the propagation of 18th - 20th generations.

Key words: Phalaenopsis, Tissue culture, Commercial p roduction