园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 318-320.

• 研究报告 • 上一篇    下一篇



  1. (1 山东农业大学园艺学院, 泰安271018; 2 山东农业大学植物保护学院, 泰安271018; 3 泰安市菜篮子科技园, 泰安271018)
  • 收稿日期:2004-04-01 修回日期:2004-06-21 出版日期:2005-04-25 发布日期:2005-04-25

Studies on the Changes of Endogenous Hormones during the Course of Regeneration from Allium fistulosum L. Mature Embryos Culture

Sheng Yanping1;Yang Jianping1;Zhang Song1;Qi Xianlei2;Li Xincheng3   

  1. (1College of Horticulture, Shandong Agricultural University, Tai'an 271018, China; 2College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China; 3 Tai'an Institute of Vegetable Basket, Tai'an 271018, China)
  • Received:2004-04-01 Revised:2004-06-21 Online:2005-04-25 Published:2005-04-25

摘要: 对大葱成熟胚的愈伤组织诱导及分化过程进行了观察, 并对再生过程中内源激素水平进行了测定。研究结果表明: 大葱愈伤组织诱导过程中, ZR在3 d时含量达峰值, 可能是导致6 d时增长率最高的主要原因, ABA在此过程中可能为负调控因子。GA3 在愈伤组织分化过程中的含量明显高于继代过程,说明其对大葱愈伤组织的分化有重要作用。适当高的6-BA与NAA比值有利于芽的分化, 但是细胞分裂素类浓度过高时则会对不定芽造成伤害。

关键词: 大葱, 成熟胚, 愈伤组织诱导, 分化, 内源激素

Abstract: The callus induction and p lant regeneration from Allium fistulosum L. mature embryos culture were studied in A. f istulosum L. and the endogenous hormone changes in this course were analyzed. The callus growth rate reached the highest point at the 6 th day after culturing, which might be caused by the maximum concentration of ZR at the 3 rd day. ABA was a negative regulation factor in the callus induction. The concentration of GA3 during callus induction wasmuch higher than that in the multip lication course, which indicated GA3 was very important in the differentiation period. The appropriate proportion between 6-BA and NAA was beneficial to the shoot differentiation of A. fistulosum L. , but there was side effect to shoot regeneration if CTK concentration was too high.

Key words: Allium fistulosum L., Mature embryos, Callus induction, Differentiation, Endogenous hormones