园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 699-702.

• 研究论文 • 上一篇    下一篇


张树珍 汤火龙 杨本鹏 刘飞虎2   

  1. ( 中国热带农业科学院热带作物生物技术国家重点实验室,海口571101; 云南大学生命科学学院,昆明650091)
  • 收稿日期:2003-04-17 修回日期:2003-06-11 出版日期:2003-12-25 发布日期:2003-12-25

Genetic Transformation of Carnation with Its ACC Oxida~ Antisense Gene

Zhang Shuzhen , Tang Huolong , Yang Benpeng ,an d Liu Feihu。   

  1. ( National研Biotechnology Laboratoryfor Tropical Crops,Chinese Academy ofTropic Agricultural Sciences,Haikou 571101,China;Co//ege of Sc/ence,Yuamm Un/,~rshy,/(ammh~650091,China)
  • Received:2003-04-17 Revised:2003-06-11 Online:2003-12-25 Published:2003-12-25

摘要: 在建立康乃馨从愈伤组织到完整植株的高频再生体系的基础上,用花特异表达启动子驱动的康乃馨ACC氧化酶反义基因通过农杆菌介导法对康乃馨进行遗传转化,获得了3株转基因植株.经多重PCR及PCR-Southem杂交检测,证实康乃馨ACC氧化酶的反义基因已整合进康乃馨的基因组中。

关键词: 康乃馨, ACC氧化酶, 花特异表达启动子, 反义基因, 遗传转化

Abstract: The high frequency regeneration system of carnation from leaf to callus to plantlets was established.
Then genetic transformation of the carnation with the ACO an tisense gene.which controled by flower-specific expression
promoter(PchsA)was performed via agrobacterium mediated method and obtained 3 of transgenic plants
via the pathway of regeneration plantlets from callus at last.Th e resuhs of the PCR and PCR.Southem hybridization
analysis showed that the carnation ACC oxidase antisense gene Was integrated into carnation genomes.It will be a
good foundation for improvement the longevity of carnation flowers.

Key words: Carnation, ACC oxidase, Antisense gene, Genetic transformation