https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2003, Vol. 30 ›› Issue (4): 485-486.

• 研究报告 • 上一篇    下一篇

基因组荧光原位杂交区分百合回交一代的不同基因组*

周树军   

  1. (山东农业大学林学院,泰安271018)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-08-25 发布日期:2003-08-25

Discrimination of the Genomes in BC1 Progeny of Asiatic Lfly and Oriental LilyUsing GISH

Zhou Shujun
  

  1. (College ofForesty,Shandong Agricultural University,Ta’an 271018,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-08-25 Published:2003-08-25

摘要: 利用东方百合(Oriental Lily)基因组DNA作荧光原位杂交的探针,用鲱鱼精子DNA或亚洲百
合(Asiatic Lily)基因组DNA作封阻DNA,对东方百合和亚洲百合杂种的回交一代(Bc )的中期染色体进行了荧光染色。该方法将东方百合和亚洲百合的两个基因组在其回交一代中清楚地区分开来。表明基因组荧光原位杂交技术既能有效地鉴定杂交的成败,又能科学地分析基因组之间染色体的交换和重组。
关键词:中图分类号:

关键词: 百合, 亚洲百合, 东方百合, 基因组荧光原位杂交, 核型

Abstract: Genomic in situ hybIridization(GISH)was used to discriminate the diferent genomes in the BC1 progeny of Oriental lily and Asiatic lily. We used gen c DNA of Oriental Lily as probe an d HS sperm DNA or genomic DNA of Asiatic Lily as block DNA for chromosome painting in the present paper. 卟e result clearly showed the diference between the two genomes of Oftental Lily an d Asiatic Lily in their BC1.This confirmed that GISH could be used not only to identify the successfulness of the cross between Oriental Lily and Asiatic Lily but also to analyze the chromosome crossover or recombination between the two diferent genomes.

Key words: Asiatic Lily, Oriental Lily, Genomic in situ hybridization(GISH), Iryotype

中图分类号: