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园艺学报 ›› 2001, Vol. 28 ›› Issue (6): 570-571.

• 研究报告 • 上一篇    下一篇

正交设计在黄金鱼花组织培养中的应用

李 艳;王 青;李英慧;王火旭   

  1. (辽宁师范大学生物系, 大连116029)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-12-25 发布日期:2001-12-25

Application of Orthogonal Design in Tissue Culture of Nematanthus glabra

Li Yan , Wang Qing , Li Yinghui , and Wang Huoxu   

  1. ( Department of Biology , Liaoning Normal University , Dalian 116029)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-12-25 Published:2001-12-25

摘要: 用正交设计法研究了4 种激素对黄金鱼花叶片诱导愈伤组织的影响。结果显示,诱导黄金鱼花愈伤组织产生的最佳激素配比为: MS + 2 ,4-D 1 mg/L + 6-BA 1 mg/L + NAA 1 mg/
L , 有2 ,4-D 的培养基中愈伤组织诱导率明显高于没有2 ,4-D 的培养基, IBA 对愈伤组织的诱导有抑制作用。

关键词: 黄金鱼花, 组织培养, 正交设计

Abstract: Effects of 2 ,4- , 6-BA , NAA and IBA on callus induction of Nematanthus glabra was studied by L9(34) orthogonal design. The experimental results showed that the best medium for callus induction was MS with 2 ,4-D 1 mg/L , 6-BA 1 mg/L , NAA 1 mg/L. Callus induction rate was higher in the mediumwith 2 ,4-D than without it , callus induction was inhibited with IBA.

Key words: Nematanthus glabra, Tissue culture, Orthogonal design

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