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园艺学报 ›› 2017, Vol. 44 ›› Issue (4): 777-783.doi: 10.16420/j.issn.0513-353x.2016-0913

• 研究报告 • 上一篇    下一篇

新疆、甘肃和河南部分地区甜瓜瓜类蚜传黄化病毒分子变异初步分析

吴 洋,刘莉铭,彭 斌,古勤生*   

  1. (中国农业科学院郑州果树研究所,郑州 450009)
  • 出版日期:2017-04-25 发布日期:2017-04-25

Preliminary Studies on Molecular Variation of Cucurbit aphid-borne yellows virus from Xinjiang,Gansu and Henan

WU Yang,LIU Liming,PENG Bin,and GU Qinsheng*   

  1. (Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China)
  • Online:2017-04-25 Published:2017-04-25

摘要:

从新疆鄯善县、甘肃瓜州县和河南通许县采集表现瓜类蚜传黄化病毒(Cucurbit aphid-borne yellows virus,CABYV)症状的甜瓜叶片样品63份,利用反转录PCR(RT-PCR)检测,从阳性样品中选取25个分离物扩增出1.4 kb的片段,并对其序列进行分析。结果表明:63份样品中,36份为CABYV阳性;获得的序列包括部分3′端的依赖RNA的RNA聚合酶(RdRp)基因、非编码区(NCR)和全长外壳蛋白(CP)基因。随机选取25个分离物的CP基因与GenBank中的序列进行比对,其序列相似性为93.2% ~ 100%。通许分离物间序列相似性为98.8% ~ 99.8%,瓜州分离物间序列相似性为98.2% ~ 100%,鄯善分离物间序列相似性为99.2% ~ 100%,组内表现出极高的同源性。基于其部分RdRp基因、NCR及CP基因序列构建的系统进化树表明:25个CABYV分离物与中国及周边地区(泰国、韩国等)的分离物聚为一簇,而与欧洲地区分离物距离较远,说明了该病毒分子变异与分离物的地理分布有关。

关键词: 甜瓜, 瓜类蚜传黄化病毒, 分子多样性, 序列比对分析

Abstract:

Cucurbit aphid-borne yellows virus(CABYV)causes yellowing symptom in cucurbit crops and distributes widely in China. Sixty-three samples exhibiting yellowing symptom were collected from Shanshan of Xinjiang,Guazhou of Gansu,and Tongxu of Henan in China. Among them,36 were positive to CABYV detected by RT-PCR. A 1.4 kb fragment consisting of partial RNA-dependent RNA polymerase(RdRp),intergenic NCR and complete coat protein(CP)gene was amplified by RT-PCR from 25 positive samples. Blast analysis showed that the fragments have high similarities of 93.2%–100% with other CABYV isolates from GenBank. The nucleotide sequence identities of CP gene were 99.2%–100% for Shanshan isolates,98.2%–100% for Guazhou isolates,98.8%–99.8% for Tongxu isolates. Phylogenetic trees based on partial RdRp,NCR and CP genes showed that the 25 isolates had a very close relationship with other isolates from China and other countries neighboring China including ThailandSouth Korea etc, but a distant relationship with European isolates,suggesting that CABYV variation appeared to be associated with its geographical distribution.

Key words: cucurbit, Cucurbit aphid-borne yellows virus, molecular diversity, sequence alignment

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