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园艺学报 ›› 2016, Vol. 43 ›› Issue (9): 1834-1838.doi: 10.16420/j.issn.0513-353x.2016-0032

• 研究报告 • 上一篇    下一篇

异源三倍体百合为母本的杂交后代GISH分析

王 红1,2,高婷婷1,辛昊阳1,戴兆霞1,席梦利1,*   

  1. (1南京林业大学林学院,南方现代林业协同创新中心,南京 210037;2江苏省农业科学院蔬菜研究所,南京 210014)
  • 出版日期:2016-09-25 发布日期:2016-09-25

GISH Analysis of the Progenies from Allotriploid Lily as Female Parent

WANG Hong1,2,GAO Ting-ting1,XIN Hao-yang1,DAI Zhao-xia1,and XI Meng-li1,*   

  1. (1Co-Innovation Center for the Sustainable Forestry in Southern China,College of Forestry,Nanjing Forestry University,Nanjing 210037,China;2Institute of Vegetable Science,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
  • Online:2016-09-25 Published:2016-09-25

摘要:

百合异源三倍体是渐渗育种的常用亲本。为了评价异源三倍体‘Royal Lace’的育种价值,采用基因组原位杂交技术分析了‘Royal Lace’及其与亚洲百合‘Pollyanna’的6个杂种后代的基因组组成。研究结果显示:‘Royal Lace’含有24条亚洲百合染色体和12条麝香百合染色体,为异源三倍体,包括1条亚洲百合重组染色体和1条麝香百合重组染色体。6个杂种后代均为非整倍体,染色体数27 ~ 32,不同杂种后代的重组染色体及重组位点存在差异,杂种后代基因组组成的不同是后代表型变异的遗传基础,因此,‘Royal Lace’是百合渐渗育种及非整倍性育种的良好亲本。

关键词: 百合, 异源三倍体, 非整倍体, GISH

Abstract:

Allotriploid lily is a common parent for introgression breeding,in order to evaluate the breeding value of the allotriploid lily‘Royal Lace’,we analyzed the genome composition of‘Royal Lace’and 6 progenies from‘Royal Lace’בPollyanna’using genomic in situ hybridization. The results showed that‘Royal Lace’is allotriploid,which consists of 24 Asiatic chromosomes and 12Longiflorumchromosomes,including 1 recombinant Asiatic chromosome and 1 recombinant Longiflorumchromosome. The 6 progenies are all aneuploids,the number of chromosomes of the progenies ranges from 27 to 32. There are differences in recombination chromosomes and break points of different progenies. The different genome composition of the progenies is the genetic basis of morphological variations. Therefore,‘Royal Lace’is a promising parent for introgression breeding and aneuploidy breeding in Lilium.

Key words: Lilium, allotriploid, aneuploid, genomic in situ hybridization

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