铁线莲‘Multi-Blue’不定芽及体细胞胚发生的初步研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 465-468.

铁线莲‘Multi-Blue’不定芽及体细胞胚发生的初步研究

张启香;方炎明*;吕梅;陈娜

(南京林业大学森林资源与环境学院,南京 210037)

收稿日期:2006-10-30 修回日期:2007-03-12 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 方炎明

A Preliminary Study on Induction of Adventitious Buds and Embryogenesis in Clematis‘Multi-Blue’

ZHANG Qi-xiang, FANG Yan-ming*,Lü Mei,and CHEN Na

(College of Forestry Resources and Environment, Nanjing Forestry University, Nanjing，Jiangsu 210037,China)

Received:2006-10-30 Revised:2007-03-12 Online:2007-04-25 Published:2007-04-25
Contact: FANG Yan-ming

摘要/Abstract

摘要：

用‘Multi-Blue’铁线莲的幼叶、茎尖、嫩茎作为外植体进行培养，发现外植体来源以茎尖为最优，基本培养基以低盐的1/2MS为最佳。茎尖在附加0.5 mg·L-1TDZ+0.01 mg? L-1NAA+2%蔗糖的1/2MS固体培养基上培养1个月后芽增殖率达200.8%，且幼苗长势健壮。茎尖在附加0.8 mg· L-1TDZ+0.5 mg· L-1NAA+2%蔗糖的1/2MS固体培养基上培养时可形成淡黄色块状至颗粒状愈伤，经多次继代培养后可逐渐形成胚性愈伤组织，胚状体诱导率可达60%，进而获得再生植株。

关键词: 铁线莲, 基本培养基, 不定芽, 体细胞胚

Abstract:

In a culture experiment, shoot apexes were the best explant and 1/2 MS was the best basal medium. Adventitious buds and calli were induced from shoot apexes of Clematis ‘Multi-Blue’which were cultured on solid 1/2MS medium containing 0.5 mg·L-1TDZ+0.01 mg? L-1NAA+2% sucrose and 0.8 mg·L-1TDZ+0.5 mg·L^-1NAA+2%sucrose respectively. Induction rate of buds is up to 200.8% after one month and the buds were vigorous. Somatic embryos were obtained from the grey yellow lumped and granular calli through successive transfer culture. The induction rate of somatic embryos was up to 60%, and it can be developed to normal seedlings in the same medium.

Key words: Clematis, Basic Media, Adventitious Buds, Embryogenesis

中图分类号:

S 682

张启香;方炎明;吕梅;陈娜. 铁线莲‘Multi-Blue’不定芽及体细胞胚发生的初步研究[J]. 园艺学报, 2007, 34(2): 465-468.

ZHANG Qi-xiang;FANG Yan-ming;Lü Mei;and CHEN Na. A Preliminary Study on Induction of Adventitious Buds and Embryogenesis in Clematis‘Multi-Blue’[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 465-468.

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