关键词: 百合, 抗病基因同源序列（RGA）, 枯萎病, 抗性鉴定

Abstract: Thirty-six lily cultivars and five wild species were analyzed to study their resistance to Fusarium oxysporum by scale inoculating method. The results showed that there exist the rich resistance lily resources to F. oxysporum. Most known plant disease-resistance genes include conserved domains in their encoded products domains. Ten pairs of primers were designed on the loci of these conserved domains. By polymerase chain reaction（PCR）and denatured polyacrylamide-gel electrophoresis techniques，resistance gene analogues（RGA）were amplified on 41 lily varieties. The RGA analysis of 41 varieties produced 159 amplification bands，156 of them showed polymorphic. The lily varieties were divided into 7 groups at 0.71 similarity coefficient. The resistance spectrum clustering analysis showed that varieties with similar resistance spectrum could finely fall into the same group，while the RGA-PCR clustering analysis showed that varieties with the similar genetic background could fall into the same group.General comparison of clustering analysis showed that there was no parallelism relationship in groups between two different types of the clustering. The result indicated resistance gene analog polymorphism，combining the resistance identification，could more accurately reflect their genetic background of parents，and enrich lily resistance germplasm，and breed durable resistance varieties.

Key words: lily, resistance gene analogue（RGA）, Fusarium oxysporium, resistance identification