芸薹种作物抽薹相关基因BrFLC1的CAPS标记

园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1635-1640.

芸薹种作物抽薹相关基因BrFLC1的CAPS标记

原玉香^1,2,孙日飞¹,张晓伟^2,3,武剑¹,徐东辉¹,张慧¹,和江明⁴,张延国¹,王晓武^1*

(¹中国农业科学院蔬菜花卉研究所，北京100081；²河南省农业科学院园艺研究所，郑州450002；³华中农业大学园艺林学学院, 武汉430070；⁴云南省农业科学院园艺作物研究所，昆明650205)

收稿日期:2008-05-16 修回日期:2008-10-23 出版日期:2008-11-25 发布日期:2008-11-25
通讯作者: 王晓武

A CAPS Marker Linked to Bolting Related Gene BrFLC1 in Brassica rapa

YUAN Yu-xiang^1,2,SUN Ri-fei¹, ZHANG Xiao-wei^2,3,WU Jian¹,XU Dong-hui¹, ZHANG Hui¹,HE Jiang-ming⁴,ZHANG Yan-guo¹,and WANG Xiao-wu^1*

(¹Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ²Institute of Horticulture, Henan academy of Agricultural sciences, Zhengzhou 450002, China; ³College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China;⁴Institute of Horticulture, Yunnan academy of Agricultural sciences, Kunming 650205, China )

Received:2008-05-16 Revised:2008-10-23 Online:2008-11-25 Published:2008-11-25
Contact: WANG Xiao-wu

摘要/Abstract

摘要： 以16份芸薹种作物DH系为试材，通过人工春化处理对其抽薹性状进行了鉴定，明确了材料间抽薹早晚存在显著差异；根据BrFLC1基因序列设计引物进行PCR扩增发现，所有16份材料均获得980 bp的片段。多序列比对与抽薹性的关联分析发现，材料的抽薹早晚与扩增片段的碱基变异相关联,并发现关联位点的碱基变异为限制性内切酶Mva I的识别位点；利用该酶对PCR产物酶切可以将材料明显区分开来，从而建立了BrFLC1基因的CAPS标记（命名为G-Mva I）。利用此标记对96份芸薹种作物DH材料的验证表明,该基因的分子标记结果与抽薹时间表型显著相关，用该标记可以进行分子标记辅助选择。

关键词: 芸薹种作物, 抽薹, BrFLC1基因, CAPS 标记

Abstract: Bolting time of 16 Brassica rapa accessions were investigated under artificial vernalization treatment. The result showed that there was a wide range variation of bolting time among these accessions. Polymerase Chain reaction (PCR) with a primer pair based on the BrFLC1 gene sequence generated a 980 bp fragment from all accessions. A base variation located at P3 was significantly associated with bolting time, and the base variation at this position is the recognition site of restriction enzyme Mva I. Therefore, a Cleaved Amplified Polymorphic Sequnence (CAPS) marker designated as G-Mva I was developed for this locus by digesting the amplified fragment with Mva I. The marker was verified in 96 B. rapa DH lines, displaying a significant association between the bolting phenotypes and the genotypes of G- Mva I. These results indicated that G-Mva I can be easily used for marker-assisted selection (MAS) in late bolting or bolting-resistance breeding.

Key words: Brassica rapa L.syn campestris L, bolting, BrFLC1 gene, CAPS marker

中图分类号:

S 634.3

原玉香;孙日飞;张晓伟;武剑;徐东辉;张慧;和江明;张延国;王晓武. 芸薹种作物抽薹相关基因BrFLC1的CAPS标记[J]. 园艺学报, 2008, 35(11): 1635-1640.

YUAN Yu-xiang;SUN Ri-fei;ZHANG Xiao-wei;WU Jian;XU Dong-hui;ZHANG Hui;HE Jiang-ming;ZHANG Yan-guo;and WANG Xiao-wu . A CAPS Marker Linked to Bolting Related Gene BrFLC1 in Brassica rapa [J]. ACTA HORTICULTURAE SINICA, 2008, 35(11): 1635-1640.

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