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园艺学报 ›› 2002, Vol. 29 ›› Issue (6): 505-509.

• 研究论文 •    下一篇

抗旱性不同的苹果属植物水分胁迫下核酸代谢及自由基变化

曹 慧;韩振海*;许雪峰   

  1. (中国农业大学园艺植物研究所, 北京100094)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2002-12-25 发布日期:2002-12-25

Effects of Water Stress on Nucleic Acid Metabolisms and Changes of Free Radicals in Malus

Cao Hui, Han Zhenhai, and Xu Xuefeng   

  1. (Institute for Horticultural Plants , China Agricultural University , Beijing 100094 , China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-12-25 Published:2002-12-25

摘要: 水分胁迫引起苹果属植物中抗旱性较弱的平邑甜茶和抗旱性较强的新疆野苹果幼苗叶片相对
含水量(RWC) 、DNA、RNA 含量下降, O2、H2O2 含量增高, DNase、RNase 活性上升; 其中DNA 含量下降的幅度小于RNA , DNase 活性上升的幅度小于RNase。抗旱性较强的新疆野苹果在水分胁迫下幼苗叶片的RWC、DNA、RNA 含量下降的幅度以及O2
、H2O2含量增高、DNase、RNase 活性上升的幅度均小于抗旱性较弱的平邑甜茶。水分胁迫下平邑甜茶和新疆野苹果幼苗叶片的DNA、RNA 含量与RWC、O2、H2O2 含量
和DNase、RNase 活性间均呈显著相关。

关键词: 苹果属, 水分胁迫, 核酸代谢, 自由基

Abstract: Effects of water stress on nucleic acid metabolisms and changes of free radicals in Malus hupehensis pamp Reld. and Malus sieversii (Ledeb) Roem. seedings were studied with polyethylene glycol (PEG6000) treatment . Under water stress , the contents of RWC , DNA and RNA in leaves decreased , while the content of O2 and H2O2, the activities of DNase and RNase increased. Compared with RNA , the less decrement of DNA content was
accompanied with higher activity of DNase. The changing extents in RWC , active oxygen , DNA , RNA , DNase and RNase in the drought tolerant variety M. sieversii were lower than the drought resistant variety M. hupehensis. Under water stress , there are high significant correlation between DNA , RNA content and DNase , RNase , RWC ,O2, H2O2 in M. hupehensis pamp Reld. and M. sieversii (Ledeb) Roem. seedings.

Key words: Malus, Water stress, Nucleic acid metabolism, Free radicals

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