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园艺学报 ›› 2008, Vol. 35 ›› Issue (11): 1685-1688.

• 研究简报 • 上一篇    下一篇

基于ITS序列的红山茶组植物系统发育关系的研究

田 敏1*,李纪元1,倪 穗1,2,范正琪1,李辛雷1   

  1. 1中国林业科学研究院亚热带林业研究所,浙江富阳 311400;2宁波大学生命科学与生物工程学院,浙江宁波 315211)
  • 收稿日期:2008-06-11 修回日期:2008-10-13 出版日期:2008-11-25 发布日期:2008-11-25
  • 通讯作者: 田 敏

Phylogenetic Study on Section Camellia Based on ITS Sequences Data

TIAN Min1*;LI Ji-yuan1;NI Sui1,2;FAN Zheng-qi1;and LI Xin-lei1
  

  1. (1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang, Zhejiang 311400, China; 2College of Life Science and Biotechnology , Ningbo University, Ningbo, Zhejiang 315211,China)
  • Received:2008-06-11 Revised:2008-10-13 Online:2008-11-25 Published:2008-11-25
  • Contact: TIAN Min

摘要:

测定了红山茶组30个物种的ITS序列,利用茶作为外类群,应用PAUP程序中的最大简约法构建了该组植物的系统发育关系。结果表明,大部分红山茶组植物构成一个单系群,这一单系群又分为两个分支。其中一个分支包括了分布在我国中南地区的大花红山茶、多齿红山茶以及分布在华东南的浙江红山茶、山茶等,其自展支持率为79 % (Clade I);另一个分支主要包括分布在我国西南地区的西南红山茶、金沙江红山茶、滇山茶等,自展支持率为92 % (Clade II)。根据ITS系统树并结合物种的形态特征及地理分布探讨了红山茶组植物的种间关系及其系统进化。

关键词: 山茶属, 红山茶组, ITS序列, 系统发育

Abstract:

Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA from 30 taxa of section Camellia were sequenced and analyzed. Taking C. sinensis as an outgroup, phylogenetic analysis of ITS sequences was conducted by using PAUP4.0. The results indicated that the most plant in section Camellia formed a monophyletic group. This group could be divided into two main clades (clade I and clade II). The clade I included the species distributed in central south and southeast of China, supported by bootstrap value of 79 %. The species distributed in southwest China formed another clade with bootstrap value of 92 %. The taxonomic and evolutional relationships in section Camellia were discussed based on the phylogenetic analysis of ITS sequences, morphological characters and geographical distribution.

Key words: Camellia, Section Camellia, ITS sequence, phylogeny

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