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园艺学报 ›› 2007, Vol. 34 ›› Issue (1): 135-140.

• 蔬菜 • 上一篇    下一篇

利用中国香瓜与哈密瓜的F2 群体构建SRAP连锁遗传图谱

王建设1*
; 姚建春1,2 ;刘 玲1 ; 王永健1 ; 李 唯2
  

  1. (1 国家蔬菜工程技术研究中心, 北京100089; 2 甘肃农业大学, 兰州730070)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-25 发布日期:2007-02-25

Construction of a Molecular Genetic Map for Melon ( Cucumis melo L. )Based on SRAP

WANG Jian-she1*
; YAO Jian-chun1,2 ; LIU Ling1 ; WANG Yong-jian1 ; LI Wei2
  

  1. (1 National Engineering Research Center for Vegetables, Beijing 100089, China; 2 Gansu Agricultural University, Lanzhou730070, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

摘要: 以中国甜瓜地方品种自交系4G21 (香瓜) 和3A832 (哈密瓜) 杂交产生的114个F2单株为
材料, 利用29对SRAP引物构建甜瓜分子遗传图谱, 共产生187个多态性位点, 其中152个位点组成12个
连锁群, 该图谱覆盖基因组长度2 077.1 cM, 平均图距13.67 cM。每个连锁群的标记数6~32个, 平均图
距9.72~19.19 cM, 连锁群长度85.3~469.1 cM。

关键词: 甜瓜, SRAP, 遗传图谱

Abstract: A molecularmap formelon (Cucumis melo L. ) was constructed with SRAP markers using a
population consisting of 114 F2 individuals derived from the cross of 4G21 (C. melo var. chinensis) and
3A832 (C. melo var. saccherinus). Twenty-nine primer pairswere used and produced 187 polymorphic loci.
The map consists of 12 linkage groups, which include 152 genetic markers, and covers 2 077.1 cM with an
average genetic distance of 13.67 cM. Every linkage group has 6 - 32 genetic markers, average genetic dis
tance is 9.72 - 19.19 cM, and the length of linkage group is 85.3 - 496.1 cM.

Key words: Melon, Cucumis melo L., SRAP, Molecular map