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园艺学报 ›› 2020, Vol. 47 ›› Issue (10): 1956-1968.doi: 10.16420/j.issn.0513-353x.2019-0680

• 研究论文 • 上一篇    下一篇

雷帕霉素预处理延缓牡丹‘洛阳红’切花衰老的生理效应

王凯轩1,3,4,王 依1,史 田1,5,丁熙柠1,袁军辉2,史国安1,*,胡永红2,*   

  1. 1河南科技大学农学院/牡丹学院,洛阳市牡丹生物学重点实验室,河南洛阳 471023;2上海辰山植物园,中国科学院上海辰山植物科学研究中心,上海 201602;3中国科学院武汉植物园,植物种质创新与特色农业重点实验室,武汉 430074;4中国科学院大学,北京 100049;5洛阳国家牡丹园,河南洛阳 471011
  • 出版日期:2020-10-25 发布日期:2020-11-10
  • 基金资助:
    国家重点研发计划项目(2018YFD1000407);国家自然科学基金项目(31372098);河南省自然科学基金项目(162300410075)

Physiological Effects of Rapamycin Pretreatment on Delaying the Senescence of Cut Flower in Tree Peony‘Luoyanghong’

Physiological Effects of Rapamycin Pretreatment on Delaying the Senescence of Cut Flower in Tree Peony‘Luoyanghong’   

  1. 1Luoyang Key Laboratory of Peony Biology,College of Agriculture/College of Tree Peony,Henan University of Science and Technology,Luoyang,Henan 471023,China;2Shanghai Chenshan Plant Science Research Center,Chinese Academy of Sciences,Shanghai Chenshan Botanical Garden,Shanghai 201602,China;3Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences,Wuhan 430074,China;4University of Chinese Academy of Sciences,Beijing 100049,China;5Luoyang National Peony Garden,Luoyang,Henan 471011,China
  • Online:2020-10-25 Published:2020-11-10

摘要: 为研究雷帕霉素靶标激酶TOR在牡丹开花衰老过程中的生理作用,以牡丹(Paeonia suffruticosa Andr.)‘洛阳红’切花为试材,用0.01 μmol • L-1雷帕霉素预处理2 h后瓶插,并测定瓶插寿命、能量物质含量、糖分含量、乙烯释放速率、呼吸速率、MDA含量以及PsTOR、PsSnRK1和PsHXK1表达量变化。结果表明:雷帕霉素预处理可延长该牡丹切花的瓶插最佳观赏期和增大花朵的最大花径;另外,雷帕霉素预处理还可提高花瓣可溶性糖含量和能荷积累,降低呼吸耗能和MDA含量,并下调瓶插初期PsSnRK1和PsHXK1的表达水平,上调瓶插后期PsTOR和PsSnRK1的表达水平。说明雷帕霉素可通过TOR途径调控能量感知,延缓牡丹切花的开放和衰老进程,进而提高瓶插品质。

关键词: 牡丹, 切花, TOR, 雷帕霉素, 能量状态, 花朵开放, 衰老

Abstract: In order to reveal effects of TOR on opening and senescence of tree peony cut flowers and its physiological mechanism,we studied vase life,energy substances content,sugar content,ethylene production rate,respiratory rate,MDA content and PsTOR,PsSnRK1 and PsHXK1 relative expression level using cut flowers of potted tree peony‘Luoyanghong’under the pretreatment of 0.01 μmol • L-1 rapamycin for 2 h. The results indicated that rapamycin increased the best viewing period and the maximum flower diameter,increased soluble sugar content and energy charge in the petals,decreased the respiration consumption and MDA content,down-regulated expression of PsTOR,PsSnRK1 and PsHXK1 genes in the early period,and up-regulated expression of PsTOR and PsSnRK1 genes in the later periods. The data suggest that rapamycin can regulate energy-aware through TOR pathway to delay the florescence and senescence of cut tree peony flowers,thus improving the quality of cut tree peony flowers during vase periods.

Key words: Paeonia suffruticosa, cut flower, TOR, rapamycin, energy status, flower opening, senescence

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