Abstract:

Cold shock domain proteins（CSPs）are cold induced proteins which play a critical role for the adaptation of plant to low-temperature stress. They are widely distributed in bacteria，plants and animals. In this study，we have cloned a Cold shock domain protein gene AmCSDP（GenBank accession number KU744005）from Ammopiptanthus mongolicus. Bioinformatics analysis showed that the ORF of AmCSDP is 540 bp，encoding 180 amino acids. The AmCSDP secondary structure consists of 8.9% α-helix，47.8% β-floding and 43.3% random coil and contains an N-terminal CSD domain. Phylogenetic analysis showed that AmCSDP shared high similarity to the homologous gene（GenBank accession number XP_016170210.1）from Arachis ipaensis. To further confirm the functionof AmCSDP，eukaryotic expression vector Pcambia2300-35S-AmCSDP-OCS was constructed and transformed into Nicotiana   

Key words: Ammopiptanthus mongolicus, cold shock domain protein gene AmCSDP, transgene, cold tolerance