Abstract: A substracted cDNA library was constructed by using SSH technique to enrich related genes.cDNA p repared from red root peel of‘Tsuda’turnip was used as tester, and that from white oneswas used as driver. 960 positive clones were got from the library. The inserted size was between 300 and 800 base pair as it was determined by PCR method. After sequencing and clustering, 302 unique geneswere obtained. By running blastn p rogram of NCB I, 99 ESTs were found to have the homologous genes of the database with known
function, and 203 ESTs without remarkable homology or with unknown.

Key words: Turnip, SSH, Subtracted cDNA library, Differentially expressed gene