Research of Programmed Cell Dea th Under Wa ter Stress in Malus robustaRehd. and Malus hupehensis ( Pamp. ) Rehd.

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (4): 469-474.

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Research of Programmed Cell Dea th Under Wa ter Stress in Malus robustaRehd. and Malus hupehensis ( Pamp. ) Rehd.

CAO Hui^1,2,3, LI Chun-xia^3,4,WANG Xiao-wei², ZOU Yan-mei¹,and SHU Huai-rui^1*

( ¹Institute of Horticultural Science and Engineering, Shandong Agricultural University, Taipan, Shandong 271018, China;²Weifang University, W eifang, Shandong 261061, China; ³Shanx i Agricultural University, Taigu, Shanx i 030801, China;⁴Yanan Vocational and Technical College, Yanan, Shaanx i 716000, China)

Received:2008-10-14 Revised:2009-04-01 Online:2009-04-25 Published:2009-04-25
Contact: SHU Huai-rui

Abstract

Abstract: The seedling of Malus robusta Rehd. and Malus hupehensis ( Pamp. ) Rehd. were treated with 20% PEG 6000. The study was to observe p rogrammed cell death under water stress through morphocytology and DNA ladder. The nuclearmorphology was observed after DAP I staining. And the results showed that the characteristic morphological changes of chromatin /chromosome and nuclear such as chromatin condensed, karyon shinked, distorted and degraduated were found with stress time. Meantime, DNA ladder was found in cells after agarose gel electrophoresis. Cell death induced bywater stress showed typ icalmorphological and biochemical p roperties of p rogrammed cell death, and thiswas an active cell death. Additionally, PCD in Malus robusta Rehd. was longer than in Malus hupehensis ( Pamp. ) Rehd. seedlings.

Key words: Malus, water stress, programmed cell death, DAPI, DNA ladder

CLC Number:

S 661.1

CAO Hui;;LI Chun-xia;WANG Xiao-wei;ZOU Yan-mei;and SHU Huai-rui. Research of Programmed Cell Dea th Under Wa ter Stress in Malus robustaRehd. and Malus hupehensis ( Pamp. ) Rehd.[J]. ACTA HORTICULTURAE SINICA, 2009, 36(4): 469-474.

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Research of Programmed Cell Dea th Under Wa ter Stress in Malus robustaRehd. and Malus hupehensis ( Pamp. ) Rehd.

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