Abstract: Infected plant seedlings of macadamia showing concentric chlorotic ring spot or brown
lethal spot symptoms were found in a nursing base located at the altitude of about 800 meters in Lincang，
Yunnan，China. In order to confirm the presence of virus，typically infected leaves of Macadamia sp. were
detected by using electron microscopy and DAS-ELISA. Virions with a diameter of about 80–85 nm，
similar in size to the Tospovirus particles，were both observed in the sap and ultrathin sections of diseased
leaves of macadamia. The crude extracts of infected leaves had a positive reaction to the complex antibody
of Watermelon silver mottle virus（WSMoV）/Groundnut bud necrosis virus（GBNV）. These results above
indicated that macadamia was infected by a Tospovirus. It is the first report of Tospovirus infecting macadamia.

Key words: macadamia, Tospovirus, ultrathin section, DAS-ELISA