Abstract: The interspecific relationship of 16 species（including 1 variation）in Lycoris was analyzed using trnH-psbA sequences. The results showed that the length of trnH-psbA sequences of the species was 546–641 bp，GC content of them was 35.86%–36.41%. There were 14 variable sites and 5 information sites，which could be used to identify some species of Lycoris. The clustering showed that Lycoris could be classified into 3 clades. The classification was basically consistent with that of morphology except for L. chinensis，L. rosea and L. aurea. This paper suggested that trnH-psbA could be used as a good molecular marker to identify or classify plants of Lycoris.

Key words: Lycoris, trnH-psbA sequence, nucleotide variation, interspecific relationship, China