Abstract: Solanine was extracted from peel of a potato variety ‘Zhong Shu 3’ by using microwave and ethanol/acetic acid solvent. Crude extract was further purified and then qualified by HPLC. The optimized extraction parameters were obtained through a 4 factor, 3 level orthogonal experiment. The results showed that the use of microwave not only increased the extraction efficiency but also reduced the extraction time. The best extraction procedure obtained from the orthogonal experiment was first to mix peel powder with ethanol/acetic acid (100/10,V/V) solvent at the ratio of 10 g of powder to 200 g·ml^-1 solvent, and then subject the mix to microwave irradiation for 6 min at the power level of 540 W.

Key words: Solanum tuberosum L., solanine, extraction, microwave-assisted extraction, high-performance liquid chromatography