Gene Expression Profiling in Response to Drought Stress in Citrus Leaves by cDNA-AFLP

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (3): 417-424.

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Gene Expression Profiling in Response to Drought Stress in Citrus Leaves by cDNA-AFLP

XIAO Jin-ping，CHEN Jun-wei，ZHANG Hui-qin，XU Hong-xia，WANG Hui-liang，and XIE Ming*

（Horticulture Institute，Zhejiang Academy of Agricultural and Sciences，Hangzhou 310021，China）

Received:2010-10-28 Revised:2011-01-27 Online:2011-03-25 Published:2011-03-25
Contact: XIE Ming

Abstract

Abstract: Transcripts involved in drought stress in‘Amakusa’tangor（Citrus reticulata × C. sinensis）were identified by cDNA-AFLP gene expression profiling in leaves under normal watering and water deficit condition. A total of 113 differentially expressed transcript-derived fragments（TDFs）were identified by selective PCR amplification using 81 primer combinations. The TDFs included 71（63%）upregulated，29（26%）downregualted，and 13（11%）transiently expressed genes. Seventy TDFs were recovered，sequenced，and functionally annotated using sequence homology to GenBank entries. Out of the 70 TDFs，33 are homolog to genes with a known function，18 are similar to genes with unknown function，and 11 have not sequence homology to GenBank entries. The function of homologous genes included signal transduction，energy metabolism，ion channel，and protein synthesis.

CLC Number:

S 666

XIAO Jin-ping;CHEN Jun-wei;ZHANG Hui-qin;XU Hong-xia;WANG Hui-liang;and XIE Ming. Gene Expression Profiling in Response to Drought Stress in Citrus Leaves by cDNA-AFLP[J]. ACTA HORTICULTURAE SINICA, 2011, 38(3): 417-424.

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Gene Expression Profiling in Response to Drought Stress in Citrus Leaves by cDNA-AFLP

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