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ACTA HORTICULTURAE SINICA ›› 2002, Vol. 29 ›› Issue (1): 57-59.

• 研究报告 • Previous Articles     Next Articles

Identification of Stenospermocarpy Mutants of Litchi by RAPD Analysis

Liu Chengming1 ,2 and Mei Mantong2
  

  1. (1 Department of Horticulture South China Agricultural University, Guangzhou 510642; 2 Genetic Engineering Laboratory , South China Agricultural University, Guangzhou 510642)
  • Received:1900-01-01 Revised:1900-01-01 Online:2002-02-25 Published:2002-02-25

Abstract: For understanding the mechanismof stenospermocarpy mutation in litchi (Litchi chinensis Sonn. ) , RAPD analysis was conducted in this study. Huaizhi and Sanyuehong , two cultivars grown in South China, and nine spontaneous mutants from them were used as materials. After screening 500 102mer random primers for polymor phism among these two cultivars and their mutants , only one prime , OPL212 , was able to generate different elec
trophoresis pattern between Huaizhi and its two seed2abortive mutants , as well as Sanyuehong and its four seed abortive mutants. The size of amplified fragments specifically for seed2abortive Huaizhi mutants was 1 645 bp , and
which for seed2abortive Sanyuehong was 722 bp. The two specific amplified bands had been considered as markers of seed abortion genes in litchi.

Key words: Litchi, Seed2abortive mutant, RAPD

CLC Number: