Abstract:

Pollen viability and the process of fertilization inClivia miniata Regel are studied by means of storing the pollen at low temperature and routine paraffinembedded sectioning technique, so as to offer some information for its cross breeding.The results are as follows: The setting percentage of fresh pollen is 35%, it declines to 22% when the pollens are kept for 70 days at -21℃, it turns to 4% when the pollens are stored for another 70 days. Two sperms have the characteristic of heteromorphism. In mature embryo sac, secondary nucleus lies in the antipodal cells end. Asynchronization, unfertilization and abortion in the process of ovule development are main reasons for low setting percentage. Fertilization and duration of each stage: Pollen grains germinate about two hours after artifical pollination, then the pollen tube prolongs in style for about 12 h. Fusion of male and female sexual cells takes place 42-50 h after pollination. The division of primary endosperm nucleus takes place 44-46 h after pollination. 6-7 days after pollination, zygote begins to devide. The dormancy stage of zygote lasts about 4-5 days.

Key words: Clivia miniata, Pollen viability, Crosspollination, Setting percentage, Fertilization