Abstract: Using the shoot—tips as the explant，the tube propagation of Prunus pseudocerasus was studied. The optimal asepsis and culture condition were established by a statistical method. The explant was asepticized as follows: 70％ ethanol 30 seconds→ 0.1％ benzalkonium bromide solution 15 minutes→0
.1％ HgCl₂ 3 minutes, besides 50 mg/L carbenicillin must be added to the media．The optimal culture condition as follows：(1)the medium for primary propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L，； (2)the medium for multiple propagation contained F14+6-BA0.5 mg/L+IAA 1.0 mg/L+GA30.5 mg/L， 2％sugar and 0.5％ sorbitol．The results also showed that diferent explants． the length of shoot-tip and the application of anti-oxidant could afect the surviving rate．

Key words: Prunus pseudocerasus, Shoot-tip culture, Tube propagation