Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase fromHami Melon

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (8): 1177-1183.

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Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase fromHami Melon

DING Qun-ying^1,3;ZHANG Rui²;LIAO Xin-fu2*;GUO Ai-guang^1,3*

(¹College of Life Science, NorthwestA & F University, Yangling, Shaanxi 712100, China; ²Xinjiang Developm ent and Research Center of Grapes and Melons, Shanshan, Xinjiang 838201, China; ³ Shaanxi Key Laboratory of Molecular Biology forAgriculture,Northwest A & F University, Yangling, Shaanxi 712100, China)

Received:2009-04-20 Revised:2009-06-23 Online:2009-08-25 Published:2009-08-25
Contact: GUO Ai-guang

Abstract

Abstract: Specific primers were designed based on the reported partial sequence of melon (Cucumis melo L. ) ACS3 gene in the GenBank to clone the full-length cDNA of ACS3 gene in using RT-PCR and RACE methods. Its GenBank Accession No. is FJ383171. The length of Hamimelon ACS gene cDNA is 1 895 bp,
this gene encodes 482 amino acids, the putative molecularweight is 54.12 kD and its pI is 8.45. The phylogenetic trees constructed by software MEGA4 showed that ACS3 gene of hamimelon was closely related to that of Cucumis sativus. The cloning of ACS3 gene full-length cDNA from the Hami melon provides the basis for understanding the role of ACS3 gene in melon.

Key words: Hamimelon, ACC synthase, RACE, sequence analysis

CLC Number:

DING Qun-ying;ZHANG Rui;LIAO Xin-fu;GUO Ai-guang;. Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase fromHami Melon[J]. ACTA HORTICULTURAE SINICA, 2009, 36(8): 1177-1183.

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Cloning and Sequence Analysis of cDNA Encoding for ACC Synthase fromHami Melon

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