Detection of Three Lily Viruses by Multiplex RT-PCR

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 443-448.

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Detection of Three Lily Viruses by Multiplex RT-PCR

XU Rong-xue^1,2,MING Jun^1*,MU Ding¹,LIU Chun¹,TANG Geng-guo²,and WANG Xiao-wu¹

(¹Institute of Vegetables and Flowers, CAAS, Beijing 100081,China; ²Nanjing Agricultural University, College of Forest Resources and Environment,Nanjing，Jiangsu 210037, China)

Received:2006-07-10 Revised:2007-03-14 Online:2007-04-25 Published:2007-04-25
Contact: MING Jun

Abstract

Abstract: A multiplex RT-PCR method was developed to simultaneously detect three viruses of Lily by using 3 sets of specific primers designed according to CMV (Cucumber mosaic virus), LMoV (Lily mottle virus) and LSV (Lily symptomless virus). All samples infected by CMV，LMoV and LSV could be amplified by multiplex PCR, and yielding three special bands of CMV(657 bp), LMoV (428 bp) and LSV(171 bp). Sequence identities of the three amplified virus fragments correlated well with other isolates from Asia and the Netherlands registered in the GenBank. Remarkably high homology in the coat protein sequences of the three viruses has been observed respectively despite their different origins.

Key words: Multiplex RT-PCR, Virus Detection, Cucumber mosaic virus, Lily mottle virus, Lily symptomless virus

CLC Number:

S 682.2

XU Rong-xue;MING Jun;MU Ding;LIU Chun;TANG Geng-guo;and WANG Xiao-wu. Detection of Three Lily Viruses by Multiplex RT-PCR[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 443-448.

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Detection of Three Lily Viruses by Multiplex RT-PCR

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