Effects of Organic Fertilizer Replacement on Soil Nutrients in Tea Garden and Yield-quality of Tea Plant

doi:10.16420/j.issn.0513-353x.2022-0481

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (10): 2207-2219.doi: 10.16420/j.issn.0513-353x.2022-0481

• Cultivation Physiology & Biochemistry • Previous Articles Next Articles

Effects of Organic Fertilizer Replacement on Soil Nutrients in Tea Garden and Yield-quality of Tea Plant

SHU Zaifa, JI Qingyong, SHAO Jingna, ZHENG Shenghong, ZHOU Huijuan, HE Weizhong^*()

Lishui Academy of Agriculture and Forestry Sciences，Lishui，Zhejiang 323000，China

Received:2023-03-31 Revised:2023-07-31 Online:2023-10-25 Published:2023-10-30

Abstract

Abstract:

The effect of soil nutrients and tea yield and quality to different fertilization structures was studied by using the‘Jingbai 1’tea cultivar as experimental material through five fertilization treatments: no fertilization，conventional fertilization，100% chemical fertilizer fertilization，30% organic fertilizer substitution and 100% organic fertilizer substitution. The results of three-year experiment showed that fertilization treatments significantly increased the contents of soil organic matter，total nitrogen (N)，available phosphorus (P)，available potassium (K) and available magnesium (Mg)，and increased the absorption of N，P，K and Mg. Whereas organic fertilizer substitution treatment showed divergent variation in nutrient absorption. Meanwhile，fertilization treatments displayed a positive effect on tea yield. 30% organic fertilizer substitution treatment revealed the highest increase in tea yield with a 27.28% average increase in the three years. In addition，the components of tea quality showed diverse responses to different treatments. When compared with no fertilization，other fertilization treatments promoted the accumulation of free amino acids，decreased the polyphenol/amino acids. After three years，compared to no fertilization treatment，other fertilization treatments decreased the contents of GA，EC，EGCG，EC，ester catechin，Non-ester catechin and total catechin. Furthermore，conventional fertilization and 30% organic fertilizer substitution treatments increased the caffeine content compared with no fertilization treatment. Meanwhile，fertilization treatment increased the content of umami amino acid，organic fertilizer substitution treatment increased obviously. In summary，the results showed that 30% organic fertilizer substitution treatment can effectively improve the tea yield and quality.

Key words: fertilization structure, soil nutrient, tea yield, tea quality

SHU Zaifa, JI Qingyong, SHAO Jingna, ZHENG Shenghong, ZHOU Huijuan, HE Weizhong. Effects of Organic Fertilizer Replacement on Soil Nutrients in Tea Garden and Yield-quality of Tea Plant[J]. Acta Horticulturae Sinica, 2023, 50(10): 2207-2219.

Figures/Tables 9

References 36

[1]	Chen Yuzhen, Wang Feng, Wu Zhidan, Zhang Wenjin, Weng Boqi, You Zhiming. 2020. Effects of chemical fertilizer reduction on yield,quality,fertilizer utilization efficiency and economic benefit of Oolong tea. Journal of Tea Science, 40 (6):758-770. (in Chinese)
	陈玉真, 王峰, 吴志丹, 张文锦, 翁伯琦, 尤志明. 2020. 化肥减施对乌龙茶产量、品质和肥料利用率及经济效益的影响. 茶叶科学, 40 (6):758-770.
[2]	Duan Y, Shen J Z, Zhang X L, Wen B, Ma Y C, Wang Y H, Fang W P, Zhu X. 2019. Effects of soybean-tea intercropping on soil-available nutrients and tea quality. Acta Physiologiae Plantarum, 41 (8):1-9. doi: 10.1007/s11738-018-2785-6
[3]	Gu S S, Hu Q L, Cheng Y Q, Bai L Y, Liu Z H, Xiao W J, Xiao W J, Gong Z H, Wu Y N, Feng K, Deng Y, Tan L. 2019. Application of organic fertilizer improves microbial community diversity and alters microbial network structure in tea(Camellia sinensis)plantation soils. Soil & Tillage Research,195 (C):104356-104365.
[4]	Guo Long, Li Chen, Liu Peishi, Chang Junfeng, Zhou Xiaotian, Zhang Ning, Zhu Zunyou, Weng Yawei, Ma Youhua. 2021. Effects of replacing chemical fertilizer with cow manure organic fertilizer on tea yield,quality,and soil fertility in tea garden. Journal of Soil and Water Conservation, 35 (6):264-269. (in Chinese)
	郭龙, 李陈, 刘佩诗, 常珺枫, 周晓天, 张宁, 祝尊友, 翁亚伟, 马友华. 2021. 牛粪有机肥替代化肥对茶叶产量、品质及茶园土壤肥力的影响. 水土保持学报, 35 (6):264-269.
[5]	Islam S, Hamid F S, Amin K, Sumreen S -uz-Zaman Q, Khan N, Khan A, Shah B H. 2017. Effect of organic fertilizer on the growth of tea(Camellia sinensis L.). International Journal of Sciences: Basic and Applied Research(IJSBAR), 36 (8):1-9.
[6]	Jiang Jingjing. 2017. Effects of excessive fertilization on soil microbial community structure[M. D. Dissertation]. Shenyang: Shenyang Agricultural University. (in Chinese)
	姜晶晶. 2017. 过量施肥对土壤微生物群落结构的影响[硕士论文]. 沈阳: 沈阳农业大学.
[7]	Kong Xiaojun, Shang Xiaoyang, Li Yusheng, Shi Yujuan, Kong Fanxin, Sheng Pingping, Wang Hui, Hui Haibin, Ding Deen. 2021. Effect of reducing chemical fertilizer and supplementing with organic fertilizer on tea yield and quality. Journal of Shandong Agricultural Sciences, 53 (11):94-99. (in Chinese)
	孔晓君, 尚晓阳, 李玉胜, 时玉娟, 孔凡鑫, 盛萍萍, 王慧, 惠海滨, 丁德恩. 2021. 减少化肥配施有机肥对茶叶产量和品质的影响. 山东农业科学, 53 (11):94-99.
[8]	Li Hai-lin, Wang Li-yuan, Cheng Hao, Wei Kang, Ruan Li, Wu Li-yun. 2017. The effects of nitrogen supply on agronomic traits and chemical componenets of tea plant. Journal of Tea Science, 37 (4):383-391. (in Chinese)
	李海琳, 王丽鸳, 成浩, 韦康, 阮丽, 吴立赟. 2017. 氮素水平对茶树重要农艺性状和化学成分含量的影响. 茶叶科学, 37 (4):383-391.
[9]	Liu Shuang. 2014. Study on chemical components and discriminant model of fresh taste of green tea[M. D. Dissertation]. Beijing: Chinese Academy of Agricultural Sciences. (in Chinese)
	刘爽. 2014. 绿茶鲜爽味的化学成分及判别模型研究[硕士论文]. 北京: 中国农业科学院.
[10]	Mao Ya-Lin, Wang Fang, Yin Jun-feng, Xu Yong-quan. 2020. Quality analysis of Tencha Made from different tea cultivars. Journal of Tea Science, 40 (6):782-794. (in Chinese)
	毛雅琳, 汪芳, 尹军峰, 许勇泉. 2020. 不同茶树品种碾茶的品质分析. 茶叶科学, 40 (6):782-794.
[11]	Martinetti L, Paganini F. 2006. Effect of organic and mineral fertilisation on yield and quality of zucchini. Acta HorticulturaeVolume,(700):125-128.
[12]	Mei Yu, Liang Xiao. 2022. Analysis of tea production and domestic sales in China in 2021. China Tea, 44 (4):17-22. (in Chinese)
	梅宇, 梁晓. 2022. 2021年中国茶叶生产与内销形势分析. 中国茶叶, 44 (4):17-22.
[13]	Pan Muhua. 2018. Effects of sheep raising on grass damage control in mountain tea gardens:a case study of Zhejiang Qier Tea Industry Co., LTD. Journal of Tea Science, 44 (2):81-83. (in Chinese)
	潘慕华. 2018. 山区茶园养羊对茶园草害控制的效应——以浙江奇尔茶业有限公司为例. 茶叶, 44 (2):81-83.
[14]	Qiu S L, Wang L M, Huang D F, Lin X J. 2014. Effects of fertilization regimes on tea yields,soil fertility,and soil microbial diversity. Chilean Journal of Agricultural Research, 74 (3):333-339. doi: 10.4067/S0718-58392014000300012 URL
[15]	Ruan Jianyun, Ma Lifeng, Yi Xiaoyun, Shi Yuanyuan, Ni Kang, Liu Meiya, Zhang Qunfeng. 2020. Integrated nutrient management in tea planation to reduce chemical fertilizer and increase nutrient use efficiency. Journal of Tea Science, 40 (1):85-95. (in Chinese)
	阮建云, 马立锋, 伊晓云, 石元值, 倪康, 刘美雅, 张群锋. 2020. 茶树养分综合管理与减肥增效技术研究. 茶叶科学, 40 (1):85-95.
[16]	Shu Zaifa, Zheng Shenghong, Shao Jingna, Zhou Huijuan, Ji Qingyong, Liu Yu, He Weizhong, Wang Liyuan. 2022. Responses of different tea cultivars(strains)to half fertilization. Journal of Tea Science, 42 (2):277-289. (in Chinese)
	疏再发, 郑生宏, 邵静娜, 周慧娟, 吉庆勇, 刘瑜, 何卫中, 王丽鸳. 2022. 不同茶树品种(系)对减半施肥的响应研究. 茶叶科学, 42 (2):277-289.
[17]	Siddiqui Y, Islam T M, Naidu Y, Meon S. 2011. The conjunctive use of compost tea and inorganic fertiliser on the growth,yield and terpenoid content of Centella asiatica(L.) urban. Scientia Horticulturae, 130 (1):289-295. doi: 10.1016/j.scienta.2011.05.043 URL
[18]	Wang J, Zhang B B, Tian Y, Zhang H C, Cheng Y, Zhang J B. 2018. A soil management strategy for ameliorating soil acidification and reducing nitrification in tea plantations. European Journal of Soil Biology, 88:36-40. doi: 10.1016/j.ejsobi.2018.06.001 URL
[19]	Wang Jieqiong, Wang Guoqing, Du Yanyu, Zhang Yu, Zhai Yunzhong, Yin Xueyan. 2017. Effects of fertilization methods on quality of white tea in Tianmu Lake area of Liyang. Journal of Zhejiang Agricultural Sciences, 58 (10):1700-1702. (in Chinese)
	王洁琼, 王国庆, 堵燕钰, 张宇, 翟云忠, 殷雪琰. 2017. 施肥方式对溧阳天目湖地区白茶品质的影响. 浙江农业科学, 58 (10):1700-1702.
[20]	Wang Ziteng, Geng Yuanbo, Liang Tao, Hu Xuedi. 2018. Effects of reducing fertilizer and organic fertilizer combination on tea garde soil and tea yield and quality. Journal of Ecology and Environment, 27 (12):2243-2251. (in Chinese)
	王子腾, 耿元波, 梁涛, 胡雪荻. 2018. 减施化肥和配施有机肥对茶园土壤养分及茶叶产量和品质的影响. 生态环境学报, 27 (12):2243-2251. doi: 10.16258/j.cnki.1674-5906.2018.12.009
[21]	Yan Zhen, Huang Jianyao, Gao Lu, Wang Dengliang. 2020. Research progress in caffeine biological metabolism in tea plant. China Tea, 42 (7):1-7. (in Chinese)
	闫振, 黄健垚, 高路, 王登良. 2020. 茶树体内咖啡碱生物代谢研究进展. 中国茶叶, 42 (7):1-7.
[22]	Yang Haoyu, Liu Huijian, Zhang Naiming, He Ting, Deng Hong, Bao Li. 2020. Effects of different fertilization application reduction methods on tea garden soil nutrients and tea quality. Journal of Southern Agriculture, 51 (4):887-896. (in Chinese)
	杨浩瑜, 刘惠见, 张乃明, 贺婷, 邓洪, 包立. 2020. 化肥减施处理对茶园土壤养分及茶叶品质的影响. 南方农业学报, 51 (4):887-896.
[23]	Yang Qinglin, Yang Xiangde, Ji Lingfei, Ma Lifeng, Ruan Jianyun. 2020. Effects of fertigation on young tea plant growth and nutrient absorption. Journal of Tea Science, 40 (1):96-104. (in Chinese)
	杨清霖, 杨向德, 季凌飞, 马立锋, 阮建云. 2020. 滴灌施肥对幼龄茶树生长和养分吸收的影响. 茶叶科学, 40 (1):96-104.
[24]	Ye Fabao, Liu Huiping, Pan Mingjian, Xia Jinying. 2020. Ecological tea garden practice and exploration of“Raising sheep to control grass and fertilizer garden”. Journal of Tea Science, 46 (1):41-43. (in Chinese)
	叶发宝, 刘慧平, 潘明俭, 夏金英. 2020. “养羊控草肥园”的生态茶园实践与探索. 茶叶, 46 (1):41-43.
[25]	Ye Qiuping. 2007. Effects of fertilizer on tea plant growth and tea quality. Tea Science and Technology,(1):26-28. (in Chinese)
	叶秋萍. 2007. 肥料对茶树生长和茶叶品质的影响. 茶叶科学技术,(1):26-28.
[26]	Yin Xue-Yan, Wang Jie-qiong, Wang Guo-Qing, Zhang Yu, Zhai Yun-zhong, Du Yan-yu. 2017. Effects of fertilization on yield and quality of white tea. Hunan Agricultural Sciences, 9:43-45,48. (in Chinese)
	殷雪琰, 王洁琼, 王国庆, 张宇, 翟云忠, 堵燕钰. 2017. 施肥技术对白茶产量和品质的影响. 湖南农业科学, 9:43-45,48.
[27]	Yin Xia, Zhou Ling-yun,Huang Jing,Zhang Shu-guang,Bao Xiao-cun. 2017. Research progress on effects of fertilization on soil fertility and tea quality in tea garden. Tea Communications, 44 (4):8-12. (in Chinese)
	银霞, 周凌云, 黄静, 张曙光, 包小村. 2017. 施肥对茶园土壤肥力及茶叶品质影响研究进展. 茶叶通讯, 44 (4):8-12.
[28]	Zhang Guorong, Li Jumei, Xu Minggang, Gao Jusheng, Gu Siyu. 2009. Effects of long-term fertilization on rice yield and soil fertility. Scientia Agricultura Sinica, 42 (2):543-551. (in Chinese)
	张国荣, 李菊梅, 徐明岗, 高菊生, 谷思玉. 2009. 长期不同施肥对水稻产量及土壤肥力的影响. 中国农业科学, 42 (2):543-551.
[29]	Zhang Junwei, Wang Zhiwen, Ji Lingfei, Guo Junjie, Ling Ning, Guo Shiwei. 2021. Effects of organic fertilizer instead of chemical fertilizer on tea production in tea gardens with different production modes. Journal of Nanjing Agricultural University, 44 (1):127-135. (in Chinese)
	张峻伟, 王志文, 季凌飞, 郭俊杰, 凌宁, 郭世伟. 2021. 有机肥替代化肥对不同生产模式茶园茶叶生产的影响. 南京农业大学学报, 44 (1):127-135.
[30]	Zhang Lifeng, Liu Jianping. 2018. Breeding report of a new albino tea variety‘Jingbai 1’. Journal of Tea Science, 44 (3):125-129. (in Chinese)
	张丽芬, 刘建平. 2018. 白化茶树新品种‘景白1号’选育报告. 茶叶, 44 (3):125-129.
[31]	Zhang L P, Li M H, Li X, Yan P, Zhang L, Han W Y. 2021. Summer pruning improves the branch growth and tea quality of tea trees(Camellia sinensis). Acta Physiologiae Plantarum, 43 (4):1-12. doi: 10.1007/s11738-020-03172-3
[32]	Zhang S N, Sun L T, Wang Y, Fan K, Xu Q S, Li Y S, Ma Q P, Wang J G, Ren W M, Ding Z T. 2020a. Cow manure application effectively regulates the soil bacterial community in tea plantation. BMC Microbiology, 20 (1):190-199. doi: 10.1186/s12866-020-01871-y
[33]	Zhang X, Luo J L, Zhang C, Chen S X. 2020b. The effects of three fertilization treatments on soil fertility and yield and quality of fresh leaves in tea gardens. Materials Science Forum, 5993:153-159.
[34]	Zhang Yu. 2012. Effects of different fertilization structures on tea yield and quality and soil nutrients in tea garden[M. D. Dissertation]. Nanjing: Nanjing Agricultural University. (in Chinese)
	张瑜. 2012. 不同施肥结构对茶叶产量品质和茶园土壤养分的影响[硕士论文]. 南京: 南京农业大学.
[35]	Zhou Xiaotian, Liu Peishi, Chang Junfeng, Guo Long, Guo Liang, Zhang Qiang, Ma Youhua. 2022. Effects of pig manure replacing chemical fertilizer on tea yield,quality and soil fertility. Chinese Journal of Soil Science, 53 (2):413-420. (in Chinese)
	周晓天, 刘佩诗, 常珺枫, 郭龙, 郭亮, 张强, 马友华. 2022. 猪粪替代化肥对茶叶产量、品质和土壤肥力的影响. 土壤通报, 53 (2):413-420.
[36]	Zhou Zhi, Liu Yang,Zhang Liming,Xu Ruineng,Sun Lili,Liao Hong. 2019. Soil nutrient status in Wuyi tea Region and its effects on tea quality-related constituents. Scientia Agricultura Sinica, 52 (8):1425-1434. (in Chinese) doi: 10.3864/j.issn.0578-1752.2019.08.012
	周志, 刘扬, 张黎明, 许锐能, 孙丽莉, 廖红. 2019. 武夷茶区茶园土壤养分状况及其对茶叶品质成分的影响. 中国农业科学, 52 (8):1425-1434. doi: 10.3864/j.issn.0578-1752.2019.08.012

肥料Fertilizer	pH	有机质Organic matter	N	P₂O₅	K₂O	Mg
羊粪Sheep manure	7.9	27	0.714	0.219	0.316	0.0303
菜籽饼Rape seed cake	7.1	68	3.350	1.120	1.290	0.5970

肥料Fertilizer	pH	有机质Organic matter	N	P₂O₅	K₂O	Mg
羊粪Sheep manure	7.9	27	0.714	0.219	0.316	0.0303
菜籽饼Rape seed cake	7.1	68	3.350	1.120	1.290	0.5970

施肥处理 Fertilization treatment	肥料及施肥量/（kg · hm^-2） Fertilizer and application amount						各元素施用量/（kg · hm^-2）
施肥处理 Fertilization treatment	羊粪 Sheep manure	菜籽饼 Rapeseed cake	尿素 Urea	过磷酸钙 Superphosphate	氯化钾 KCl	茶叶专用肥 Tea special fertilizer	N	P₂O₅	K₂O	Mg
不施肥No fertilization	0	0	0	0	0	0	0	0	0	0
^a常规Conventional	2 250	2 250	750	600	125		428.94	82.63	126.14	14.11
^b化肥Chemical（C）			114			1 125	298.80	90.00	135.00	11.81
^c C70% + O30%	12 855		79.5			787.5	300.82	91.16	135.13	12.16
^d有机肥Organic（O）	42 857						306.00	93.86	135.43	12.99

施肥处理 Fertilization treatment	肥料及施肥量/（kg · hm^-2） Fertilizer and application amount						各元素施用量/（kg · hm^-2）
施肥处理 Fertilization treatment	羊粪 Sheep manure	菜籽饼 Rapeseed cake	尿素 Urea	过磷酸钙 Superphosphate	氯化钾 KCl	茶叶专用肥 Tea special fertilizer	N	P₂O₅	K₂O	Mg
不施肥No fertilization	0	0	0	0	0	0	0	0	0	0
^a常规Conventional	2 250	2 250	750	600	125		428.94	82.63	126.14	14.11
^b化肥Chemical（C）			114			1 125	298.80	90.00	135.00	11.81
^c C70% + O30%	12 855		79.5			787.5	300.82	91.16	135.13	12.16
^d有机肥Organic（O）	42 857						306.00	93.86	135.43	12.99

年份 Year	施肥处理 Fertilization treatment	pH	有机质/（g · kg^-1） Organic matter	全N/（g · kg^-1） Total N	P₂O₅/ （mg · kg^-1）	K₂O / （mg · kg^-1）	总Mg/（mg · kg^-1） Total Mg
2019	不施肥No fertilization	4.59 ± 0.35 b	20.85 ± 3.33 a	2.02 ± 0.17 ab	39.56 ± 3.31 b	86.54 ± 3.00 c	20.50 ± 0.66 c
	常规Conventional	4.45 ± 0.42 ab	45.94 ± 4.41 c	3.05 ± 0.24 bc	58.54 ± 5.08 c	144.60 ± 2.74 e	27.07 ± 1.91 d
	化肥Chemical（C）	4.16 ± 0.26 a	31.73 ± 2.67 b	2.78 ± 0.19 b	52.05 ± 2.49 c	102.38 ± 5.50 d	20.83 ± 2.01 c
	C70% + O30%	4.49 ± 0.31 ab	47.77 ± 3.83 cd	2.83 ± 0.30 b	58.65 ± 5.51 c	138.56 ± 3.64 e	31.45 ± 1.65 de
	有机肥Organic（O）	4.88 ± 0.45 b	55.47 ± 3.39 d	2.89 ± 0.26 b	74.62 ± 6.87 d	210.08 ± 10.34 fg	30.67 ± 1.37 de
2020	不施肥No fertilization	4.56 ± 0.39 b	18.55 ± 1.63 a	1.62 ± 0.16 a	35.07 ± 2.46 b	66.64 ± 1.46 b	15.51 ± 0.56 ab
	常规Conventional	4.32 ± 0.16 ab	55.94 ± 3.96 d	3.85 ± 0.41 c	81.95 ± 3.52 de	121.16 ± 2.60 de	22.21 ± 1.75 cd
	化肥Chemical（C）	4.16 ± 0.22 a	34.73 ± 3.66 b	2.68 ± 0.17 b	74.89 ± 5.11 d	115.30 ± 7.77 de	13.05 ± 0.40 ab
	C70% + O30%	4.45 ± 0.39 ab	52.77 ± 3.54 d	3.13 ± 0.30 bc	84.73 ± 4.30 de	146.38 ± 8.21 e	17.72 ± 1.55 abc
	有机肥Organic（O）	4.70 ± 0.25 b	59.47 ± 6.65 d	2.99 ± 0.40 bc	91.90 ± 5.08 e	196.05 ± 10.01 f	19.30 ± 0.90 c
2021	不施肥No fertilization	4.51 ± 0.32 b	15.96 ± 2.58 a	1.42 ± 0.16 a	19.48 ± 1.33 a	49.23 ± 3.00 a	12.21 ± 0.30 a
	常规Conventional	4.49 ± 0.44 ab	54.94 ± 4.49 d	3.35 ± 0.34 bc	145.00 ± 3.82 g	175.00 ± 7.13 ef	32.21 ± 1.98 de
	化肥Chemical（C）	4.24 ± 0.43 a	45.73 ± 6.07 c	2.78 ± 0.09 b	111.95 ± 4.01 f	126.60 ± 3.82 de	25.05 ± 0.87 cd
	C70% + O30%	4.59 ± 0.36 b	56.77 ± 5.36 d	2.93 ± 0.19 bc	154.66 ± 10.34 g	156.46 ± 6.76 e	27.72 ± 0.22 d
	有机肥Organic（O）	4.76 ± 0.31 b	63.47 ± 3.25 d	3.39 ± 0.20 bc	188.14 ± 9.3 6h	253.33 ± 11.33 g	33.30 ± 1.80 e

Effects of Organic Fertilizer Replacement on Soil Nutrients in Tea Garden and Yield-quality of Tea Plant

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年份 Year	采摘标准 Picking standard	施肥处理 Fertilization treatment	N	P	K	Mg
2019	一芽一叶One bud one leaf	不施肥No fertilization	50.18 ± 1.06 a	5.82 ± 0.36 a	15.41 ± 0.21 a	1.76 ± 0.06 abc
		常规Conventional	52.44 ± 1.25 a	6.37 ± 0.20 cd	15.57 ± 0.30 a	1.78 ± 0.06 abc
		化肥Chemical（C）	52.81 ± 1.48 a	6.40 ± 0.17 cde	15.54 ± 0.16 a	1.76 ± 0.02 abc
		C70% + O30%	52.65 ± 1.31 a	6.43 ± 0.27 cde	15.64 ± 0.30 a	1.77 ± 0.05 abc
		有机肥Organic（O）	51.45 ± 1.33 a	6.35 ± 0.09 bcd	15.53 ± 0.33 a	1.75 ± 0.02 ab
	一芽二叶One bud two leaves	不施肥No fertilization	51.90 ± 2.40 a	5.86 ± 0.21 a	17.92 ± 0.68 b	1.71 ± 0.14 ab
		常规Conventional	53.55 ± 1.09 a	6.13 ± 0.29 abc	18.70 ± 0.43 bcd	1.70 ± 0.10 ab
		化肥Chemical（C）	52.69 ± 2.37 a	5.97 ± 0.25 ab	18.31 ± 0.84 b	1.78 ± 0.11 abc
		C70% + O30%	52.75 ± 1.80 a	6.06 ± 0.15 abc	18.26 ± 0.20 b	1.72 ± 0.04 a
		有机肥Organic（O）	51.71 ± 2.90 a	6.21 ± 0.24 abcd	17.93 ± 0.11 b	1.74 ± 0.11 ab
	单芽Simple bud	不施肥No fertilization	50.00 ± 3.20 a	6.50 ± 0.18 cde	18.36 ± 0.49 bc	1.80 ± 0.08 abc
		常规Conventional	51.67 ± 1.28 a	6.68 ± 0.24 e	19.26 ± 0.90 cde	1.95 ± 0.12 d
		化肥Chemical（C）	51.60 ± 1.13 a	6.68 ± 0.31 de	19.29 ± 0.78 cde	1.87 ± 0.10 cd
		C70% + O30%	52.03 ± 1.42 a	6.58 ± 0.45 cde	19.74 ± 0.84 e	1.85 ± 0.09 bcd
		有机肥Organic（O）	50.43 ± 1.20 a	6.61 ± 0.34 de	19.56 ± 1.17 de	1.91 ± 0.08 cd
2020	一芽一叶One bud one leaf	不施肥No fertilization	56.20 ± 1.71 a	12.09 ± 1.08 c	11.04 ± 0.67 a	1.78 ± 0.09 a
		常规Conventional	57.79 ± 1.42 a	13.66 ± 0.27 d	11.98 ± 0.13 bc	1.82 ± 0.07 ab
		化肥Chemical（C）	59.92 ± 1.52 a	14.08 ± 0.23 de	11.85 ± 0.23 bc	1.82 ± 0.03 ab
		C70% + O30%	57.80 ± 1.43 a	14.31 ± 0.40 de	11.53 ± 0.41 ab	1.82 ± 0.17 ab
		有机肥Organic（O）	56.62 ± 1.71 a	14.68 ± 0.39 e	11.72 ± 0.19 b	1.85 ± 0.08 ab
	一芽二叶One bud two leaves	不施肥No fertilization	56.65 ± 2.62 a	9.92 ± 0.13 a	13.51 ± 0.44 d	1.77 ± 0.15 a
		常规Conventional	58.35 ± 1.20 a	10.45 ± 0.39 a	14.07 ± 0.49 d	1.87 ± 0.13 ab
		化肥Chemical（C）	58.53 ± 2.60 a	9.94 ± 0.29 a	13.84 ± 0.48 d	1.85 ± 0.09 ab
		C70% + O30%	57.71 ± 1.99 a	10.44 ± 0.17 a	13.51 ± 0.48 d	1.83 ± 0.12 ab
		有机肥Organic（O）	56.80 ± 3.17 a	11.28 ± 0.26 b	13.56 ± 0.39 d	1.88 ± 0.17 abc
	单芽Simple bud	不施肥No fertilization	55.62 ± 3.43 a	15.14 ± 0.37 fg	11.25 ± 0.27 ab	1.83 ± 0.03 ab
		常规Conventional	56.12 ± 1.41 a	15.27 ± 0.21 fg	12.11 ± 0.27 bc	2.03 ± 0.03 c
		化肥Chemical（C）	57.12 ± 1.03 a	15.76 ± 0.55 ghi	11.78 ± 0.26 b	1.88 ± 0.05 abc
		C70% + O30%	57.37 ± 1.49 a	15.93 ± 0.52 hi	12.42 ± 0.36 c	1.83 ± 0.06 ab
		有机肥Organic（O）	55.87 ± 1.20 a	16.04 ± 0.47 i	11.60 ± 0.19 ab	1.97 ± 0.03 bc
2021	一芽一叶One bud one leaf	不施肥No fertilization	47.08 ± 1.95 ab	13.53 ± 1.22 c	10.34 ± 0.57 a	1.78 ± 0.10 a
		常规Conventional	48.18 ± 1.22 abc	14.92 ± 0.31 d	11.46 ± 0.20 bc	1.80 ± 0.12 ab
		化肥Chemical（C）	46.22 ± 2.11 a	15.70 ± 0.28 de	11.31 ± 0.56 bc	1.81 ± 0.09 ab
		C70% + O30%	47.07 ± 1.56 ab	15.82 ± 0.55 e	10.77 ± 0.74 ab	1.82 ± 0.11 ab
		有机肥Organic（O）	46.04 ± 1.59 a	16.32 ± 0.80 e	11.23 ± 0.64 bc	1.82 ± 0.12 ab
	一芽二叶One bud two leaves	不施肥No fertilization	49.65 ± 2.25 bcd	10.90 ± 0.30 a	13.20 ± 0.55 ef	1.74 ± 0.10 a
		常规Conventional	50.39 ± 3.72 cd	10.93 ± 0.15 a	13.49 ± 0.28 f	1.83 ± 0.09 ab
		化肥Chemical（C）	51.66 ± 0.74 d	11.46 ± 0.40 a	12.45 ± 0.60 de	1.84 ± 0.08 ab
		C70% + O30%	51.78 ± 1.17 d	11.35 ± 0.14 a	12.71 ± 0.37 ef	1.83 ± 0.12 ab
		有机肥Organic（O）	50.53 ± 1.66 cd	12.57 ± 0.34 b	12.91 ± 0.65 ef	1.86 ± 0.17 ab
	单芽Simple bud	不施肥No fertilization	45.80 ± 2.03 a	16.36 ± 0.30 ef	11.77 ± 0.56 cd	1.83 ± 0.03 ab
		常规Conventional	47.30 ± 0.82 ab	16.36 ± 0.23 ef	11.49 ± 0.18 bc	1.99 ± 0.06 c
		化肥Chemical（C）	48.13 ± 1.19 abc	17.20 ± 0.76 fg	11.22 ± 0.56 bc	1.85 ± 0.09 ab
		C70% + O30%	48.06 ± 2.20 abc	17.68 ± 0.32 g	11.00 ± 0.62 abc	1.82 ± 0.06 ab
		有机肥Organic（O）	47.64 ± 1.86 abc	17.58 ± 0.78 g	11.14 ± 0.63 abc	1.88 ± 0.03 bc

采摘标准 Picking standard	处理 Treatment	2019			2020			2021
采摘标准 Picking standard	处理 Treatment	游离氨基酸 Amino acid	茶多酚 Tea polyphenol	酚氨比 Ratio of polyphenols/ amino acids	游离氨基酸 Amino acid	茶多酚 Tea polyphenol	酚氨比 Ratio of polyphenols/ amino acids	游离氨基酸 Amino acid	茶多酚 Tea polyphenol	酚氨比 Ratio of polyphenols/ amino acids
一芽一叶 One bud one leaf	不施肥No fertilization	5.12 ± 0.90 bc	31.33 ± 1.59 ab	6.31 ± 1.43 abc	3.97 ± 0.62 bcd	32.33 ± 0.95 abcd	8.31 ± 1.42 abc	3.82 ± 0.49 bc	27.83 ± 2.04 ab	7.42 ± 1.37 bc
	常规 Conventional	6.70 ± 0.77 f	31.59 ± 4.26 ab	4.77 ± 0.85 a	4.57 ± 0.33 ef	32.78 ± 0.97 abcd	7.20 ± 0.63 a	4.29 ± 0.16 de	26.06 ± 0.73 a	6.08 ± 0.31 a
	化肥 Chemical（C）	5.65 ± 0.90 de	33.74 ± 2.81 b	6.10 ± 1.19 abc	4.28 ± 0.88 def	31.96 ± 1.49 abc	7.66 ± 1.32 ab	4.08 ± 0.21 cd	27.30 ± 0.77 ab	6.72 ± 0.52 ab
	C70% + O30%	5.80 ± 0.66 de	32.35 ± 1.48 bc	5.62 ± 0.65 ab	4.41 ± 0.31 ef	31.15 ± 0.74 a	7.08 ± 0.46 a	4.54 ± 0.35 f	27.35 ± 1.25 ab	6.05 ± 0.53 a
	有机肥 Organic（O）	5.91 ± 0.25 e	32.49 ± 1.96 ab	5.51 ± 0.54 a	4.41 ± 0.29 ef	32.07 ± 0.68 abcd	7.29 ± 0.40 a	4.66 ± 0.19 f	27.80 ± 0.61 ab	5.98 ± 0.32 a
一芽二叶 One bud two leaves	不施肥No fertilization	4.11 ± 0.16 b	33.27 ± 2.24 b	8.10 ± 0.72 d	4.13 ± 0.31 cdef	31.41 ± 2.09 ab	7.66 ± 1.13 ab	3.59 ± 0.33 abc	29.01 ± 1.78 b	8.12 ± 0.76 cd
	常规 Conventional	4.70 ± 0.36 bc	29.25 ± 1.33 a	6.26 ± 0.66 abc	4.86 ± 0.39 f	31.04 ± 1.13 a	6.43 ± 0.73 a	3.76 ± 0.39 abc	27.87 ± 1.27 ab	7.48 ± 1.02 bc
	化肥 Chemical（C）	4.36 ± 0.25 bc	35.24 ± 1.74 b	8.03 ± 0.39 d	4.58 ± 0.29 ef	31.22 ± 2.90 ab	6.82 ± 0.50 a	3.64 ± 0.35 abc	28.45 ± 0.87 b	7.89 ± 0.95 bcd
	C 70% + O 30%	4.57 ± 0.53 bc	32.18 ± 3.61 ab	7.16 ± 1.48 bcd	4.63 ± 0.16 ef	31.74 ± 1.33 ab	6.87 ± 0.40 a	3.84 ± 0.38 bc	28.61 ± 1.29 b	7.50 ± 0.74 bc
	有机肥 Organic（O）	4.53 ± 0.37 bc	33.48 ± 1.61 b	7.43 ± 0.84 cd	4.72 ± 0.43 ef	31.49 ± 1.59 ab	6.73 ± 0.94 a	3.78 ± 0.15 abc	28.58 ± 1.51 b	7.57 ± 0.48 bc
单芽 Simple bud	不施肥No fertilization	2.46 ± 0.12 a	33.75 ± 1.69 b	13.72 ± 0.38 g	2.97 ± 0.51 a	34.70 ± 2.81 de	12.05 ± 2.98 e	3.31 ± 0.29 a	28.96 ± 1.29 b	8.81 ± 0.98 d
	常规 Conventional	2.75 ± 0.15 a	32.14 ± 1.58 ab	11.74 ± 1.13 ef	3.38 ± 0.32 ab	34.74 ± 2.07 de	10.40 ± 1.67 de	3.65 ± 0.32 abc	28.20 ± 0.63 b	7.77 ± 0.73 bcd
	化肥 Chemical（C）	2.68 ± 0.17 a	34.71 ± 2.64 b	13.03 ± 1.73 fg	3.49 ± 0.56 abc	34.48 ± 1.33 cde	10.06 ± 1.66 cde	3.52 ± 0.10 ab	29.30 ± 0.90 b	8.33 ± 0.39 cd
	C 70% + O 30%	2.82 ± 0.13 a	31.69 ± 2.72 ab	11.23 ± 0.87 e	3.65 ± 0.41 abcd	33.98 ± 1.59 bcde	9.44 ± 1.51 bcd	3.72 ± 0.17 abc	29.01 ± 0.85 b	7.81 ± 0.35 bcd
	有机肥 Organic（O）	2.83 ± 0.02 a	33.77 ± 2.60 b	11.93 ± 0.84 ef	3.41 ± 0.32 ab	35.72 ± 1.28 e	10.55 ± 1.21 de	3.72 ± 0.18 abc	28.75 ± 0.98 b	7.75 ± 0.56 bcd

采摘标准 Picking standard	施肥处理 Fertilization treatment	GA		EGC		C		EC		EGCG
一芽一叶 One bud one leaf	不施肥No fertilization	0.15 ± 0.00 cd		2.38 ± 0.25 ab		5.29 ± 0.39 b		1.22 ± 0.04 abc		5.02 ± 0.17 abcd
	常规Conventional	0.15 ± 0.01 bc		2.79 ± 0.21 bcd		3.83 ± 0.33 a		1.15 ± 0.04 ab		4.74 ± 0.16 ab
	化肥Chemical（C）	0.14 ± 0.00 ab		3.03 ± 0.10 cd		4.15 ± 0.52 a		1.21 ± 0.06 abc		5.10 ± 0.44 abcd
	C70% + O30%	0.14 ± 0.01 ab		2.98 ± 0.16 cd		4.14 ± 0.11 a		1.18 ± 0.07 ab		4.97 ± 0.19 abcd
	有机肥Organic（O）	0.14 ± 0.00 a		2.96 ± 0.05 cd		4.35 ± 0.39 a		1.22 ± 0.03 abc		4.95 ± 0.06 abcd
一芽二叶 One bud two leaves	不施肥No fertilization	0.14 ± 0.01 ab		3.05 ± 0.19 cd		4.14 ± 0.94 a		1.29 ± 0.10 bc		5.57 ± 0.17 d
	常规Conventional	0.15 ± 0.01 abc		2.79 ± 0.21 bcd		3.94 ± 0.13 a		1.16 ± 0.03 ab		4.78 ± 0.13 ab
	化肥Chemical（C）	0.14 ± 0.00 a		2.85 ± 0.04 cd		4.17 ± 0.61 a		1.23 ± 0.05 abc		5.05 ± 0.19 abcd
	C70% + O30%	0.14 ± 0.01 ab		2.99 ± 0.07 cd		4.06 ± 0.92 a		1.24 ± 0.11 abc		4.99 ± 0.29 abcd
	有机肥Organic（O）	0.14 ± 0.01 ab		2.95 ± 0.17 cd		4.05 ± 0.55 a		1.19 ± 0.14 abc		4.90 ± 0.58 abc
单芽 Simple bud	不施肥No fertilization	0.17 ± 0.01 f		3.16 ± 0.60 d		5.47 ± 0.39 b		1.33 ± 0.13 c		5.49 ± 0.53 cd
	常规Conventional	0.17 ± 0.01 ef		2.33 ± 0.10 a		5.59 ± 0.05 b		1.11 ± 0.18 a		4.59 ± 0.75 a
	化肥Chemical（C）	0.16 ± 0.01 de		3.00 ± 0.51 cd		5.35 ± 0.24 b		1.11 ± 0.13 bc		5.33 ± 0.55 bcd
	C70% + O30%	0.16 ± 0.01 de		2.64 ± 0.54 cd		5.56 ± 0.45 b		1.29 ± 0.05 bc		5.32 ± 0.21 bcd
	有机肥Organic（O）	0.14 ± 0.01 ab		3.13 ± 0.18 d		4.16 ± 0.50 a		1.22 ± 0.05 abc		5.30 ± 0.40 bcd
采摘标准 Picking standard	施肥处理 Fertilization treatment		ECG		酯型儿茶素 Ester catechin		非酯型儿茶素 Non-ester catechin		总儿茶素 Total catechin
一芽一叶 One bud one leaf	不施肥No fertilization		3.79 ± 0.16 c		8.88 ± 0.28 bcd		8.81 ± 0.32 cd		17.69 ± 0.53 cd
	常规Conventional		2.40 ± 0.19 a		7.77 ± 0.24 a		7.14 ± 0.25 a		14.91 ± 0.45 a
	化肥Chemical（C）		2.38 ± 0.39 a		8.39 ± 0.55 abc		7.48 ± 0.80 ab		15.87 ± 0.33 abc
	C70% + O30%		2.67 ± 0.16 a		8.30 ± 0.20 abc		7.64 ± 0.15 ab		15.95 ± 0.32 abc
	有机肥Organic（O）		2.50 ± 0.09 a		8.53 ± 0.41 abc		7.45 ± 0.13 ab		15.97 ± 0.51 abc
一芽二叶 One bud two leaves	不施肥No fertilization		2.80 ± 0.43 ab		8.48 ± 1.20 abc		8.37 ± 0.58 bcd		16.85 ± 1.71 bc
	常规Conventional		2.40 ± 0.10 a		7.89 ± 0.10 ab		7.19 ± 0.14 a		15.07 ± 0.08 ab
	化肥Chemical（C）		2.58 ± 0.23 a		8.25 ± 0.61 abc		7.63 ± 0.32 ab		15.88 ± 0.93 abc
	C70% + O30%		2.49 ± 0.34 a		8.29 ± 1.09 abc		7.48 ± 0.62 ab		15.77 ± 1.69 ab
	有机肥Organic（O）		2.60 ± 0.53 a		8.19 ± 0.71 abc		7.50 ± 1.01 ab		15.69 ± 1.37 ab
单芽 simple bud	不施肥No fertilization		3.83 ± 0.26 c		9.96 ± 0.43 e		9.32 ± 0.78 d		19.28 ± 1.20 d
	常规Conventional		3.19 ± 0.73 b		9.03 ± 0.64 cde		7.77 ± 1.48 ab		16.80 ± 1.92 bc
	化肥Chemical（C）		3.76 ± 0.37 c		9.64 ± 0.43 de		9.09 ± 0.90 cd		18.73 ± 1.29 d
	C70% + O30%		3.90 ± 0.21 c		9.49 ± 0.44 de		9.22 ± 0.17 d		18.70 ± 0.59 d
	有机肥Organic（O）		2.77 ± 0.15 ab		8.51 ± 0.62 abc		8.07 ± 0.50 abc		16.58 ± 0.99 abc

采摘标准 Picking standard	处理 Treatment	天冬氨酸 Aspartic	谷氨酸 Glutamic	丝氨酸 Serine	丙氨酸 Alanine	茶氨酸 Theanine	甲硫氨酸 Methionine	脯氨酸 Proline
一芽一叶 One bud one leaf	不施肥No fertilization	0.06 ± 0.02 abcd	0.26 ± 0.01 bcd	0.09 ± 0.00 ab	0.17 ± 0.08 e	1.60 ± 0.16 a	0.01 ± 0.00 ab	0.17 ± 0.14 a
	常规Conventional	0.06 ± 0.02 abcd	0.28 ± 0.02 cde	0.11 ± 0.01 abc	0.18 ± 0.03 e	1.71 ± 0.07 abc	0.01 ± 0.01 ab	0.15 ± 0.03 a
	化肥Chemical（C）	0.05 ± 0.01 ab	0.28 ± 0.01 cde	0.10 ± 0.01 abc	0.15 ± 0.02 cde	1.70 ± 0.03 abc	0.01 ± 0.00 a	0.15 ± 0.04 a
	C 70% + O 30%	0.06 ± 0.02 abcd	0.31 ± 0.02 cde	0.11 ± 0.02 abc	0.16 ± 0.04 de	1.71 ± 0.02 bc	0.01 ± 0.00 a	0.27 ± 0.11 b
	有机肥Organic（O）	0.05 ± 0.01 abd	0.34 ± 0.04 fg	0.10 ± 0.01 abc	0.16 ± 0.01 de	1.77 ± 0.07 c	0.01 ± 0.00 a	0.27 ± 0.07 b
一芽二叶 One bud two leaves	不施肥No fertilization	0.05 ± 0.01 abc	0.27 ± 0.07 cde	0.13 ± 0.05 c	0.14 ± 0.03 bcde	1.60 ± 0.04 a	0.02 ± 0.01 b	0.15 ± 0.03 a
	常规Conventional	0.07 ± 0.03 abcd	0.31 ± 0.05 def	0.09 ± 0.01 abc	0.10 ± 0.02 abc	1.61 ± 0.13 a	0.01 ± 0.00 ab	0.13 ± 0.03 a
	化肥Chemical（C）	0.04 ± 0.01 a	0.25 ± 0.01 abc	0.11 ± 0.00 abc	0.10 ± 0.03 abc	1.62 ± 0.02 ab	0.01 ± 0.00 ab	0.15 ± 0.02 a
	C 70% + O 30%	0.06 ± 0.02 abcd	0.20 ± 0.04 a	0.12 ± 0.03 abc	0.08 ± 0.05 a	1.80 ± 0.04 c	0.02 ± 0.00 ab	0.16 ± 0.03 a
	有机肥Organic（O）	0.07 ± 0.01 d	0.22 ± 0.01 ab	0.12 ± 0.04 abc	0.10 ± 0.02 abc	1.74 ± 0.04 bc	0.02 ± 0.00 ab	0.16 ± 0.02 a
单芽 Simple bud	不施肥No fertilization	0.06 ± 0.01 abcd	0.32 ± 0.04 ef	0.08 ± 0.01 a	0.10 ± 0.02 abc	1.63 ± 0.15 ab	0.01 ± 0.01 ab	0.13 ± 0.04 a
	常规Conventional	0.07 ± 0.01 bcd	0.38 ± 0.03 gh	0.10 ± 0.02 abc	0.09 ± 0.03 ab	1.64 ± 0.09 ab	0.01 ± 0.00 a	0.15 ± 0.03 a
	化肥Chemical（C）	0.05 ± 0.01 ab	0.38 ± 0.05 gh	0.12 ± 0.03 abc	0.12 ± 0.03 abcd	1.61 ± 0.02 a	0.01 ± 0.00 ab	0.13 ± 0.02 a
	C 70% + O 30%	0.07 ± 0.01 cd	0.42 ± 0.05 hi	0.12 ± 0.03 bc	0.10 ± 0.01 abc	1.80 ± 0.03 c	0.02 ± 0.00 ab	0.16 ± 0.02 a
	有机肥Organic（O）	0.10 ± 0.00 e	0.44 ± 0.02 i	0.13 ± 0.02 c	0.11 ± 0.02 abc	1.72 ± 0.03 bc	0.02 ± 0.00 ab	0.16 ± 0.02 a

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[2]	WANG Xiaofang1,2,，XIANG Kun2,，WANG Yanfang3，LI Qianjin4，JIANG Weitao1，SHENG Yuefan1，WANG Haiyan1，CHEN Xuesen1，YIN Chengmiao1，and MAO Zhiquan1,**. Effects of Tagetes erecta Biofumigation on Apple Replanted Soil Environment and Physiological Characteristics of Malus hupehensis Seedlings [J]. ACTA HORTICULTURAE SINICA, 2019, 46(12): 2383-2396.
[3]	CHEN Xue-sen1,*，ZHANG Rui-jie1，WANG Yan-ting2，WANG Nan1，JIANG Sheng-hui1，XU Hai-feng1，LIU Jing-xuan1，WANG De-yun1，QU Chang-zhi1，ZHANG Yan-min1，JIANG Yuan-mao1，and MAO Zhi-quan1 . Effects of Growing Hairy Vetch（Vicia villosa）on the Soil Nutrient，Enzyme Activities and Microorganisms in Apple Orchard [J]. ACTA HORTICULTURAE SINICA, 2016, 43(12): 2325-2334.
[4]	ZHANG Dong，ZHENG Li-wei，HAN Ming-yu**，GAO Chen-xi，LUO Wen-wen，and MA Juan-juan. Studies of the Standard Range of the Soil Nutrients in Apple Orchard in Loess Plateau [J]. ACTA HORTICULTURAE SINICA, 2016, 43(1): 121-131.
[5]	FU Xue-Qin, LIU Ju-Er, HUANG Wen-Xin. Effects of Natural Grass on Soil Microbiology，Nutrient and Fruit Quality of Nanfeng Tangerine Yard [J]. ACTA HORTICULTURAE SINICA, 2015, 42(8): 1551-1558.
[6]	TANG Yu-Qin, PENG Liang-Zhi, CHUN Chang-Pin, LING Li-Li, FANG Yi-Wen, YAN Xiang. Correlation Analysis on Nutrient Element Contents in Orchard Soils and Sweet Orange Leaves in Southern Jiangxi Province of China [J]. ACTA HORTICULTURAE SINICA, 2013, 40(4): 623-.
[7]	XI Zhu-mei;LI Hua;LONG Yan;ZHANG Jin;and PANG Xue-liang. Variation of Soil Microbial Populations and Relationships Between Microbial Factors and Soil Nutrients in Cover Cropping System of Vineyard [J]. ACTA HORTICULTURAE SINICA, 2010, 37(9): 1395-1402.
[8]	PAN Xue-jun;;ZHANG Wen-e;FAN Wei-guo;PENG Gui-hua;and LUO Guo-hua;. Effects of Sod Culture and Intercropping Green Manure on the Soil Nutrient，Enzyme Activities and Microorganisms in Bonsai Citrus [J]. ACTA HORTICULTURAE SINICA, 2010, 37(8): 1235-1240.
[9]	DU She-ni;BAI Gang-shuan;. Astragalus adsurgens Is not Suitable Interplant in Armeniaca vulgaris×A. sibiricaOrchard in Loess Hilly and Gully Region [J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1135-1140.
[10]	Li_Hui-ke;Zhang Guang-Jun;Zhao Zheng-Yang;and Li Kai-Rong. Effects of herbage-interplanted on soil Nutrient of non-irrigated apple orchard in the Loess Plateau [J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 477-480.