Expression Characteristics and Functional Analysis of FvYABBY5.1 in Strawberry

doi:10.16420/j.issn.0513-353x.2021-0351

Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (7): 1458-1472.doi: 10.16420/j.issn.0513-353x.2021-0351

• Research Papers • Previous Articles Next Articles

Expression Characteristics and Functional Analysis of FvYABBY5.1 in Strawberry

CAI Jianfa¹^,², MO Xuelian²^,³, GUAN Sicong¹^,², CHEN Xu², XUE Cheng¹^,^*()

¹College of Hoticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China
²Horticultural Plant Biology and Metabolomics Center,Haixia Institute of Science and Technology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
³College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Received:2021-12-16 Revised:2022-03-07 Online:2022-07-25 Published:2022-07-29
Contact: XUE Cheng E-mail:xcheng@sdau.edu.cn

Abstract

Abstract:

A total of five YABBY genes were identified from woodland strawberry（Fragaria vesca‘Hawaii 4’）. Based on phylogenetic tree analysis of YABBY gene in rosaceous plant,those YABBY genes were divided into five subfamilies:INO,YAB2,YAB3,CRC and YAB5. Gene structure analysis showed that exon and intron number of YABBY genes were different in the five sub-family,but were insignificantly different in the same subfamily. And the dispersion of protein motifs was little difference among all YABBY members. These results suggest that the function of YABBY genes were conservative in strawberry. Expression profile analysis showed that the expression level of FvYAB5.1 gene in dormant buds and non-dormant buds of strawberry runner was significantly different. In transgenic Arabidopsis thaliana,we found that overexpression FvYAB5.1 leaded to curly leaf shape,dwarf phenotype and delayed flowering time. The expression levels of SHOOT MERISTEMLESS（STM）and KNAT2,which regulate shoot apical meristem（SAM）development,were significantly inhibited. Meanwhile,the transcriptional expression levels of auxin and cytokinin-related genes AtARF5,AtARR4 and AtIPT7 were significantly increased,while the transcriptional expression levels of AtARR7 and AtARR15 were significantly repressed. In summary,we speculate that FvYAB5.1 gene may be involved in the development of SAM in strawberry runner by regulating the antagonistic relationship between cytokinin and auxin,leading to the alternating growth of dormant and non-dormant buds in the runner.

Key words: Fragaria vesca, YABBY, runner, shoot apical meristem

CLC Number:

S668.4

CAI Jianfa, MO Xuelian, GUAN Sicong, CHEN Xu, XUE Cheng. Expression Characteristics and Functional Analysis of FvYABBY5.1 in Strawberry[J]. Acta Horticulturae Sinica, 2022, 49(7): 1458-1472.

Figures/Tables 11

References 58

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基因 Gene	引物序列（5′-3′） Primer sequence
FvActin	F:ACCGTTGATTCGCACAATTGGTCATCG;R:TACTGCGGGTCGGCAATCGGACG
FvINO	F:GTCACTGCAGTTGCCTCCTA;R:TTTCCTGCGAACCCTGATCG
FvYAB2.1	F:AATCCTGACATTAGCCATAGGG;R:CATGAGAAAATGACCATCGCTT
FvYAB3.1	F:CTGCTATGTTCACTGCAACTTT;R:CATCATGTTAGGCTGATTGGTG
FvYAB3.2	F:AAGTTTGTTCAAGACTGTGACG;R:TACTCCTCCTCTTGGTCTTACA
FvYAB5.1	F:CTTTTGCAATATTGTTCTCGCG;R:CTTTGGATGATGAACCCGAATC
AtActin	F:GTCGTACAACCGGTATTGTGCTG;R:CTCTCTCTGTAAGGATCTTCATGAGGT
AtBP	F:TCATGGAAGCATACTGTGACA;R:TGACTCAGAAGGATATGGCCA
AtSTM	F:TGTCAGAAGGTTGGAGCACCA;R:TTTGTTGCTCCGAAGGGTAA
AtKNAT2	F:GATTGCCAAAAGGTGGGAGC;R:TGTCGCCTTCAGTAGGGTA
AtARF5	F:TTATCTTCTTGTAGCCTCTT;R:CCACACTCAACACTAACT
AtARR7	F:TCAATGCCAGGACTTTCAGGA;R:TGCTCCTTCTTTGAGACATTCTTG
AtARR15	F:TCAATGCCGGGACTAACAGG;R:TGCTCCTTCTATCATACATTGTTCT
AtARR4	F:GACCAGAATCGACAGATGCCT;R:AGCTTCCGTTTGTTTCCGTT
AtIPT7	F:GGAGGAAGTGGAAGTAGAG;R:CACACATACGAAATCTTGGA

基因 Gene	引物序列（5′-3′） Primer sequence
FvActin	F:ACCGTTGATTCGCACAATTGGTCATCG;R:TACTGCGGGTCGGCAATCGGACG
FvINO	F:GTCACTGCAGTTGCCTCCTA;R:TTTCCTGCGAACCCTGATCG
FvYAB2.1	F:AATCCTGACATTAGCCATAGGG;R:CATGAGAAAATGACCATCGCTT
FvYAB3.1	F:CTGCTATGTTCACTGCAACTTT;R:CATCATGTTAGGCTGATTGGTG
FvYAB3.2	F:AAGTTTGTTCAAGACTGTGACG;R:TACTCCTCCTCTTGGTCTTACA
FvYAB5.1	F:CTTTTGCAATATTGTTCTCGCG;R:CTTTGGATGATGAACCCGAATC
AtActin	F:GTCGTACAACCGGTATTGTGCTG;R:CTCTCTCTGTAAGGATCTTCATGAGGT
AtBP	F:TCATGGAAGCATACTGTGACA;R:TGACTCAGAAGGATATGGCCA
AtSTM	F:TGTCAGAAGGTTGGAGCACCA;R:TTTGTTGCTCCGAAGGGTAA
AtKNAT2	F:GATTGCCAAAAGGTGGGAGC;R:TGTCGCCTTCAGTAGGGTA
AtARF5	F:TTATCTTCTTGTAGCCTCTT;R:CCACACTCAACACTAACT
AtARR7	F:TCAATGCCAGGACTTTCAGGA;R:TGCTCCTTCTTTGAGACATTCTTG
AtARR15	F:TCAATGCCGGGACTAACAGG;R:TGCTCCTTCTATCATACATTGTTCT
AtARR4	F:GACCAGAATCGACAGATGCCT;R:AGCTTCCGTTTGTTTCCGTT
AtIPT7	F:GGAGGAAGTGGAAGTAGAG;R:CACACATACGAAATCTTGGA

基因名称 Gene	引物序列（5′-3′） Primer sequence
FvYAB5.1	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCGAGCTGCATCGATG R:GGGGACCACTTTGTACAAGAAAGCTGGGTTAGCATAACTGGTGACAGGTACTG
FvYAB3.1	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCTGCTGCTGCATCTT R:GGGGACCACTTTGTACAAGAAAGCTGGGTTGTAGGGGGAGACGCCC
FvYAB3.2	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCATCCTCTAATTCTGCTTCTT R:GGGGACCACTTTGTACAAGAAAGCTGGGTTGTAAGGAGAGACCCGCACA

基因名称 Gene	引物序列（5′-3′） Primer sequence
FvYAB5.1	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCGAGCTGCATCGATG R:GGGGACCACTTTGTACAAGAAAGCTGGGTTAGCATAACTGGTGACAGGTACTG
FvYAB3.1	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCTGCTGCTGCATCTT R:GGGGACCACTTTGTACAAGAAAGCTGGGTTGTAGGGGGAGACGCCC
FvYAB3.2	F:GGGGACAAGTTTGTACAAAAAAGCAGGCTTAATGTCATCCTCTAATTCTGCTTCTT R:GGGGACCACTTTGTACAAGAAAGCTGGGTTGTAAGGAGAGACCCGCACA

基因 Gene	亚家族 Subfamily	基因号 Gene ID	染色体位置 Chromosome location	蛋白长度/aa Protein length	不稳定系数 Instability coefficient	等电点 pI	亲水性 Gravy
FvINO	INO	gene04115-v1.0-hybrid	scf0513158:3866711..3868355	237	46.40	5.51	-0.659
FvYAB3.1	YAB3	gene03938-v1.0-hybrid	scf0513153:189961..192371	218	47.85	8.24	-0.312
FvYAB2.1	YAB2	gene22887-v2.0.a2-hybrid	Chr03:14803114..14809010	447	62.07	10.01	-0.503
FvYAB5.1	YAB5	gene31056-v2.0.a2-hybrid	Chr01:1699533..1703162	180	45.54	8.19	-0.422
FvYAB3.2	YAB3	gene32251-v1.0-hybrid	scf0513098:214010..216501	232	45.90	8.58	-0.424

Expression Characteristics and Functional Analysis of FvYABBY5.1 in Strawberry

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