园艺学报 ›› 2009, Vol. 36 ›› Issue (3): 415-420.

• 研究简报 • 上一篇    下一篇


曹岩坡1,3;高志奎1*;何俊萍1;王 梅1;高荣孚2   

  1. 1河北农业大学园艺学院,河北保定071001;2北京林业大学生物科学与技术学院,北京100083;3河北省农林科学院经济作物研究所,河北石家庄 050051)
  • 收稿日期:2008-11-17 修回日期:2009-02-19 出版日期:2009-03-25 发布日期:2009-03-25
  • 通讯作者: 高志奎

Effects of Exogenous Salicylic Acid on Nitrate Accumulation and Reduction and Assimilation in the Leaves of Chinese chive

CAO Yan-po1,3,GAO Zhi-kui1*, HE Jun-ping1,WANG Mei1, and GAO Rong-fu2

  1. (1College of Horticulture,Agriculture University of Hebei, Baoding,Hebei 071001, China; 2College of Biological Sciences and Biotechnolog, Beijing Forestry University, Beijing 100083, China;3Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei 050051, China)
  • Received:2008-11-17 Revised:2009-02-19 Online:2009-03-25 Published:2009-03-25
  • Contact: GAO Zhi-kui


针对韭菜生产中硝酸盐累积问题,研究了叶面喷施水杨酸(SA)对韭菜硝酸盐累积及还原同化的影响。在不同氮素水平下,叶面喷施3.0 mmol·L-1SA后6~9 d可明显降低韭菜叶片硝酸盐含量15.9%~21.6%,而当SA水平达到10.0 mmol·L-1时会加重硝酸盐的积累。叶面喷施3.0 mmol·L-1SA后9 d,韭菜叶片硝酸还原酶(NR)和谷胺酰氨合成酶(GS)活性分别比对照增加了48.9%和40.3%、净光合速率(Pn)和可溶性蛋白含量分别提高了43.4%和21.3%;同时,韭菜叶片谷氨酸草酰乙酸转氨酶(GOT)活性和谷氨酸丙酮酸转氨酶(GPT)活性也均有增加。因此,叶面喷施的外源水杨酸显著提高了氮还原动力泵NR活性以及氮同化动力泵GS活性,同时调动光合作用和转氨作用的积极协同配合,促进了硝态氮转化为游离氨基酸和可溶性蛋白,减少了硝酸盐进入液泡贮积。

关键词: 韭菜, 水杨酸, 硝酸盐累积, 硝酸盐还原同化


In view of nitrate accumulation Chinese chive production, effects of exogenous salicylic acid on nitrate accumulation and reduction and assimilation in the leaves of Chinese chive were studied. The results indicated that the nitrate content of Chinese chives were significantly decreased from 15.9% to 21.6% with various concentration SA treatment after 6 to 9 days under different levels of nitrogen. Optimal concentration of SA is 3.0 mmol·L-1, and SA concentration reached 10.0 mmol·L-1, nitrate accumulation could be aggravated. After 9 days with spraying 3.0 mmol·L-1 SA on leaves, the activity of nitrate reductase (NR) and glutamine synthetase (GS) were increased 48.9% and 40.3%; photosynthetic rate and dissolvable protein were increased 43.4% and 21.3%; However, the activity of GOT and GPT were slightly higher than the control. Therefore, activity of NR (which was named as 'N-reducing-driving-pump') and GS (which was called 'first driving pump') were significantly increased by spraying on leaves with exogenous SA, while photosynthesis and transaminations were played the positive role of coordination, and translation of nitrate into free amino acid and dissolvable protein were promoted and nitrate accumulating into the vacuole of Chinese chives were reduced.

Key words: Chinese chive, salicylic acid, nitrate accumulation, nitrate reduction and assimilation