园艺学报 ›› 2009, Vol. 36 ›› Issue (10): 1531-1537.

• 研究报告 • 上一篇    下一篇

基于核ITS与叶绿体trnL-F 序列分析12种紫金牛属植物的种间关系与变异

徐玲玲1;李同建1; 张美云1;易官美2;廖亮1*   

  1. (1江西九江学院生命科学学院, 江西九江332000;2宁波城市职业技术学院, 浙江宁波315502)
  • 收稿日期:2009-06-12 修回日期:2009-08-19 出版日期:2009-10-25 发布日期:2009-10-25
  • 通讯作者: 廖亮

Interspecific Relationships and Variation of 12 Species in Ardisia Sw.(Myrsinaceae) Based on ITS and trnL-F Data Sets

XU Ling-ling1;LI Tong-jian1;ZHANG Mei-yun1;YI Guan-mei2; LIAO Liang1*   

  1. (1 College of Life Science, Jiujiang University, Jiujiang, Jiangxi 332000, China; 2 Ningbo City College of Vocational Technology, Ningbo, Zhejiang 315502, China)
  • Received:2009-06-12 Revised:2009-08-19 Online:2009-10-25 Published:2009-10-25
  • Contact: LIAO Liang

摘要: 对江西紫金牛属12种植物17个样本及外类群柳叶密花树的核ITS序列和叶绿体trnL 2F序列
进行最大简约法(MP) 和邻接法(NJ ) 分析, 两种方法得到的系统发育树基本一致。基于trnL 2F序列所建立的系统发育树将19个样本聚类成6个分支, 而基于ITS序列建立的系统发育树将18个样本聚类成2个分支, 说明紫金牛属并不是一个单系类群。基于ITS序列建立的系统发育树将腋序组(Ⅰ) 和锯齿组( Ⅲ) 聚在一起, 说明这两个组有较近的关系, 而圆齿组( Ⅱ) 稍远。基于ITS序列所建立的系统树将4个紫金牛样本聚类成2支, 说明紫金牛的群体中至少存在两个核基因组类型, 根据染色体数、ITS序列和trnL-F序列综合分析, 推测紫金牛很可能是异源四倍体。

关键词: 紫金牛属, ITS序列, trnL-F序列, 种间关系

Abstract: The nuclear encoded internal transcribed spacer(ITS) region and the plastid encoded trnL-F
region were sequenced for 17 samples from 12 species of the genus Ardisia Sw. and outgroup Rapanea lin-
earis. Based on maximum parsim ony and neighbor-joining analyses, we reconstructed the phylogenetic rela-
tionships of 19 samples of Ardisia. The two methods generated similar trees. Nineteen samples of Ardisia are
separated into six clades intrnL-F trees. Eighteen samples of Ardisia are separated into two clades in ITS
trees. The results showed that the genus Ardisia Sw. could not be amonophyletic group. The Sec.t  Akosmos
(Ñ) and Sec.t Bladhia (Ó) form a clade in ITS trees, which indicated that they were closely related.
Four samples of A. japonica form two clades in ITS trees, which showed that there were at least two types of
nuclear genome in A. japonica population. According to analysis of the chromosomes number, ITS sequences
and trnL-F sequences, A. japonicais supposed to be a allotetraploid.

Key words: Ardisia, ITS sequence;trnL-F sequence, interspecific relationship