园艺学报 ›› 2018, Vol. 45 ›› Issue (4): 669-677.doi: 10.16420/j.issn.0513-353x.2017-0448

• 研究论文 • 上一篇    下一篇


邓晓东1,焦国柱2,刘 聪1,韦同路1,郭大勇1,谢宗周1,*,刘继红1,*   

  1. 1华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070;2湖北省巴东县农业服务中心,湖北巴东 444300
  • 出版日期:2018-04-25 发布日期:2018-04-25
  • 基金资助:


Role of Exogenous Spermidine in Keeping On-tree Ponkan Fruits Fresh and Its Effect on Formation of Abscission Zone

DENG Xiaodong1,JIAO Guozhu2,LIU Cong1,WEI Tonglu1,GUO Dayong1,XIE Zongzhou1,*,and LIU Jihong1,*   

  1. 1Key Laboratory of Horticultural Plant Biology,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;2Agricultural Service Center at Badong County of Hubei Province,Badong,Hubei 444300,China
  • Online:2018-04-25 Published:2018-04-25


柑橘果实越冬留树保鲜是延长果实采收期及减缓果实集中上市压力的一个有效措施,然而椪柑果实留树贮藏研究较少。本研究中以‘新生系3号’椪柑(Citrus reticulata)为对象,分析外源亚精胺(Spermidine,Spd)在果实越冬留树保鲜中减少落果的作用及可能机制。结果表明,外源喷施1 mmol · L-1 Spd明显降低了落果率,且不影响果实品质。外施Spd降低果实离层组织中活性氧含量,纤维素酶、多聚半乳糖苷醛酸酶和果胶甲酯酶等细胞壁降解关键酶活性及其基因表达量,从而抑制离层的形成。因此Spd可以用于椪柑果实留树保鲜。

关键词: 椪柑, 留树保鲜, 亚精胺, 离层


On-tree storage is an effective strategy to keep citrus fruits fresh on tree,which can prolong harvesting season and relieve the pressure of fruit sale in a limited period. However,little knowledge is available for implementation of on-tree storage of ponkan fruits. In the present study,exogenous spermidine(Spd)was supplied to‘New Line 3’ponkan(Citrus reticulata)to investigate the role of Spd in lessening fruit dropping during overwinter on-tree storage and to understand the potential underlying mechanisms. Our results demonstrated that application of 1 mmol · L-1 Spd had negligible effects on influencing fruit quality,while noticeably decreased the rate of fruit dropping. Meanwhile,exogenous supply of Spd suppressed ROS production in the fruit abscission zone,concurrent with significant reduction of the activities and expression levels of cellulase(CX),polygalacturonase(PG)and pectin esterase(PME),three key enzymes associated with cell wall degradation. This leads to retardation of cell wall degradation and impairment of formation of abscission zone. Taken together,our data revealed that exogenous application of Spd could delay the formation of abscission zones in the fruits,allowing it to be used possibly for keeping ponkan on-tree fruits fresh overwinter.

Key words: ponkan, Citrus reticulata, on-tree storage, spermidine, abscission zone