关键词: 喀西茄, 黄萎病, WRKY转录因子

Abstract:

A total of four unigene sequences that share high homology with WRKY protein were previously obtained from the root transcriptome database of Solanum aculeatissimum．Phylogenetic analysis showed that the four WRKY transcription factors belong to IC，IIa，and III group of WRKY family，respectively．The expression profiles of these four WRKY genes in different organs（root，stem and leaf），as well as in the root of Solanum aculeatissimum of different time after Verticillium dahliae inoculation were analyzed by quantitative real-time PCR．The results showed that the expression levels in root of Unigene6502 and Unigene20920 were significant higher than those in stem and leaf，but the expression levels in leaf of CL1273.Contig2 and CL3641.Contig1 were significantly higher than those in root and stem．The four WRKY genes have different expression patterns upon Verticillium dahliae inoculation．The expression of Unigene6502 and Unigene20920 were up-regulated and then was significantly decreased，while the expression of CL1273.Contig2 and CL3641.Contig1 were up-regulated and down-regulated，respectively.

Key words: Solanum aculeatissimum, verticillium wilt, WRKY transcription factor