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园艺学报 ›› 2004, Vol. 31 ›› Issue (4): 540-542.

• 研究报告 • 上一篇    下一篇

杏黄兜兰和硬叶兜兰的种子试管培养

陈之林 叶秀 梁承邺 段 俊   

  1. (中国科学院华南植物园,广州510650)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-08-25 发布日期:2004-08-25

Seed Germination in Vitro of Paphiopedilum armeniacum and P.micranthum

Chen Zhilin,Ye Xiulin ,Liang Chengye,and Duan Jun
  

  1. (South China Botarf Garden,Academia Sinica,Guangzhou 510650,China )
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

摘要: :杏黄兜兰(Paphiopedilum armeniacum)和硬叶兜兰(P.micranthum)受粉后120 d和180 d的
种子接种到1/4 MS,Ms,R,Kc,Vw 和花宝1号培养基 进行试管培养。低盐浓度的培养基较适宜兜兰
种子萌发,R培养基效果最好,萌发率可达30%以上;果荚采收的时间对种子萌发有重要的影响;添加
200 mL.I| 的椰子乳可促进种子萌发。育苗培养基中添加活性炭2 mg·L 和香蕉匀浆100 g。I 有利于
种子苗生长。

关键词: 兜兰, 杏黄兜兰, 硬叶兜兰, 试管培养

Abstract: The seeds take from capsules harvested 1 20 days and 1 80 days after pollination of Paphiopedi—
lum armeniacum and P.micranthum.These were inoculated on 1/4MS, MS, R, KC,VW and “Hyponex
1” media.AU the germination was recorded in 120 days after pollination for both two species.The higher
germination rate get in lower salt concentration medium than higher salt concentration medium.R medium iS
the best for germ ination in test.Coconut milk stimulate germination.The addition of activated charcoal 2 g·
L‘。and banana homogenate 100 g·L iS benefit for growth and development of seedlings.

Key words: Paphiopedilum, P.armeniacum, P.micranthum, Germ ination in vitro