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园艺学报 ›› 2004, Vol. 31 ›› Issue (4): 537-539.

• 研究报告 • 上一篇    下一篇

农杆菌介导的蝴蝶兰基因转化系统的建立

柴明良 金斗焕   

  1. ( 浙江大学园艺系,杭州310029; 韩国建国大学园艺系,汉城143—701)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-08-25 发布日期:2004-08-25

Establishment of Agrobacterium-mediated Transformation in Phalaenopsis

Chai Mingliang and Kim Doohwan   

  1. ( Department of Horticulture,Zhejiang University,Hangzhou 310029,China; Department of Horticultural Science,KonkukUniversity,Seoul 143—701,Korea)
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-08-25 Published:2004-08-25

摘要: 针刺后的蝴蝶兰‘White Hikaru’的类原球茎(PLB),与含绿色荧光蛋白基因( )和潮霉
素磷酸转移酶基因(^p£)的pCAMBIA 1300一SmGFP的根瘤农杆菌LBA4404 共培养,培育出了转基因的蝴
蝶兰。经绿色荧光蛋白检测和Southern印迹,证实了再生植株中含cop基因和hpt基因。

关键词: 蝴蝶兰, 类原球茎, 农杆菌介导的转化, 绿色荧光蛋白基因, 潮霉素磷酸转移酶基因

Abstract: Transgenic orchid(Phalaenopsis CV.White Hikaru)plants were regenerated by inoculating a
needle wounded protocorm.1ike body (PLB)with Agrobacterium tumefaciens strain LBA4404 harboring
pCAMBIA 1300-SmGFP that carries green fluorescent protein gene( )and hygromycin phosphotransferase
gene( f).The expression and presence of the transgenes, and f were assessed by detection of green
fluorescent protein under microscope and Southern analysis,respectively.

Key words: Phalaenopsis, Protocorm—like body(PLB), Agrobacterium.mediated transformation, Greenfluorescent protein gene( ), Hygromycin phosphotransferase gene( f)