园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 745-747.

• 研究报告 • 上一篇    下一篇


何松林1; 杨秋生1; 孔德政1; 张启翔2; 田中道男3   

  1. ( 1河南农业大学林学园艺学院,郑州450002; 2北京林业大学园林学院,北京100083; 3日本国立香川大学农学部,香川761—0795)
  • 收稿日期:2003-05-14 修回日期:2003-08-18 出版日期:2003-12-25 发布日期:2003-12-25

Effect of Sucrose Concentration on the Growth of Oncidium Explants in Vitrounder CO2 Enrichment

He Songlin ,Yang Qiusheng ,Kong Dezheng’,Zhang Qixiang ,and Tanaka M。   

  1. ( College of Forestry and Horticulture,ttenan Agricultural University,Zhengzhou 450002,China; College of Architecture,2 Beijing Forestry University, 2g 100083,China; Faculty of Agriculture, University, Koe,aw 761—0795,Japan)
  • Received:2003-05-14 Revised:2003-08-18 Online:2003-12-25 Published:2003-12-25

摘要: 探讨了培养室内施CO2条件下,培养基中不同蔗糖浓度(30、20、10、5、2.5和0 g·L )对文心兰试管苗生长的影响.结果表明:无糖培养基中的试管苗可以以光合自养的方式正常生长,但生长速度略低;提高培养基糖浓度可以显著促进试管苗的生长,30 g·L (常规培养条件的糖浓度)与20 g·L 处理相比,株高、叶长、叶幅、根数、总鲜样质量和总干样质量等主要生长指标无明显差异,说明文心兰试管苗在施CO2培养时,培养基中的糖仍是必要的,但可以适当减少用量。

关键词: 文心兰, 二氧化碳, 气体肥料, 培养基, 含糖量, 离体培养

Abstract: Oncidium aloha ‘1wan ga’ in vitro shoots were used as plan t materials for experiments to study the efect of difierent sucrose concentrations (30,20,10,5,2.5,0 g·L )in the culture medium on the growth 0f the in vitro sho ts under CO,enrichment conditions. The results showed that the in vitro shoots had photoau totrophic capability. Under CO2 enrichm ent conditions, in vitro shots being cultivated in the sugar-free culturemed ium could grow normally by photoautotrophic capab ility,but a bit slowly. Increas ing the sucrose concentration in the culture medium could significan tly promote the growth of the in vitro shots of Oncidi. Under CO2 enrichment condition. in vitro sho ts grew be tter in the sugar culture medium than in the sugar-free culture medium .Compared th the sucrose concentration (30 g·L )un der normal culture conditions ,when the sucrose concen tration dropped to 20 g·L|。. there was not significan t difference be tween the tw o concentration treatments in the main growth indices of the in vitro shots including plant height, length of leaves, range of leaves, number of roots. total fresh mas s an d dry matter mass. The above results indicate that under C02 enrichm ent cul ture conditions. the existence of sucrose in the culture medium is still necessary for Oncidium ,but compared with normal culture methods. it can partly reduce the amount of sucrose used in the culture medium .

Key words: Oncidium, Carbon dioxide, Caseous fertilizer, Sucrose content, In vitro cullure, Medium