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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1897-1906.doi: 10.16420/j.issn.0513-353x.2018-0888

• 研究论文 • 上一篇    下一篇

‘龙岩野柿1号’雄花和两性花雌雄蕊发育的细胞形态学观察

李华威1,王丽媛2,孙 鹏2,索玉静2,韩卫娟2,买旖旎2,刁松锋2,袁德义1,*,傅建敏2,*   

  1. 1中南林业科技大学林学院,长沙 410004;2中国林业科学研究院经济林研究开发中心,国家林业局泡桐研究开发中心,郑州 450003
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2017ZA004-3,CAFYBB2017ZA005);国家自然科学基金青年科学基金项目(31500559)

Cytomorphological Observation on Development of Pistil and Stamen of Male and Hermaphrodite Floral Buds of Diospyros kaki‘Longyan Yeshi 1’

LI Huawei1,WANG Liyuan2,SUN Peng2,SUO Yujing2,HAN Weijuan2,MAI Yini2,DIAO Songfeng2,YUAN Deyi1,*,and FU Jianmin2,*   

  1. 1College of Forestry,Central South University of Forestry and Technology,Changsha 410004,China;2Non-Timber Forest Research and Development Center,China Paulownia Research Center,State Forestry Administration,Chinese Academy of Forestry,Zhengzhou 450003,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 在龙岩野柿子代群体中发现一株雄全同株个体‘龙岩野柿1号’,全株3朵合生聚伞形花序的中间1朵为两性花的概率大于80%,两侧分别为完全雄花。以‘龙岩野柿1号’的雄花和两性花为材料,采用常规石蜡切片法对其雌、雄蕊发育进行观察。结果表明:其两性花雌、雄蕊原基均发育正常,雄花雌蕊原基发育中无子房功能性组织产生;两性花发育早于雄花。雄花和两性花的花药4室,花药壁发育为双子叶型,由表皮、药室内壁、中层和腺质绒毡层组成;四分体为四面体型和对称型,减数分裂为同时型;成熟花粉为2–细胞型,3条萌发沟;单核靠边期存在少量畸形花粉粒和空花粉粒;不同药室及同一药室的小孢子发育存在不同步现象。两性花子房8室,中轴胎座,倒生胚珠,双珠被,薄珠心,单孢原细胞;大孢子四分体直线排列,功能大孢子位于合顶端,蓼型胚囊。

关键词: 柿, 雄全同株, 雄花, 两性花, 雌蕊, 雄蕊, 形态学

Abstract: An andromonoecy Diospyros kaki‘Longyan Yeshi 1’was found in the population of Longyan Yeshi. Its floral buds were three-flower cyme. The probability that the middle one was hermaphrodite floral buds was over 80%,and the two on both sides were unisexual male floral buds. It is a new excellent material for studying the regulatory mechanism of sex differentiation of Diospyros species. The developmental differences of male and hermaphrodite floral buds of‘Longyan Yeshi 1’was observed with paraffin section. The results showed that:the stamen and pistil primordia of hermaphrodite floral buds were normal. However,no ovary functional tissue was produced in the development of the pistil primordia of male floral buds. The anther of male and hermaphrodite floral buds were tetrasporangiate and the anther wall that consisted of epidermis,endotheca,middle layer and glandular tapetum was dicotyledonous development. The microspore were tetrahedral and symmetrical tetrads;Mature pollen grains were 2-cell type with three germ pores;A few abnormal pollen grain and empty pollen grain exsit at late uninucleate stage;Different or the same anther had asynchronous meiosis of microspore mother cells. The ovule of hermaphrodite floral buds had eight locules with axial placenta and anatropous ovule;The ovule was bitegmic and tenuinucellate with single spore cell. The megaspore tetrad arranged in a straight line and the functional megaspore located at the apex of the embryo sac which was polygonum type.

Key words: Diospyros kaki, andromonoecy, male floral bud, hermaphrodite floral bud, pistil, stamen, morphology

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