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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1399-1408.doi: 10.16420/j.issn.0513-353x.2018-0827

• 研究报告 • 上一篇    下一篇

忍冬花蕾延迟开花与内源激素调控研究

李建军*,连笑雅,王 兰   

  1. 河南师范大学生命科学学院,河南省道地药材保育及利用工程技术研究中心,绿色药材生物技术河南省工程实验室,河南新乡 453007
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    中医药公共卫生服务补助专项(财社〔2017〕66号);河南省企业技术创新引导专项项目(172107000031)

Study on the Regulation Mechanism of Endogenous Hormones in Delayed Flowering of Lonicera japonica

LI Jianjun*,LIAN Xiaoya,and WANG Lan   

  1. College of Life Science,Henan Normal University,Green Medicine Biotechnology Henan Engineering Laboratory,Engineering Technology Research Center of Nursing and Utilization of Genuine Chinese Crude Drugs in Henan Province,Xinxiang,Henan 453007,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 为研究激素(IAA、ZR、GA3、ABA)对忍冬(Lonicera japonica Thunb.)花期的调控机理,以忍冬主栽品种‘大毛花’和新品种‘特蕾1号’为试验材料,观察单花各发育时期花色、形态和花期时长的变化,并采用酶联免疫法检测4种内源激素的变化动态。结果表明,‘特蕾1号’各时期花均比‘大毛花’大,且二白期、大白期和凋花期均比‘大毛花’长,尤其是大白期长达12 d,而‘大毛花’仅为1 d。在整个花期内,‘特蕾1号’和‘大毛花’ZR和GA3含量均表现为先高后低的趋势,ABA含量均表现为逐渐上升的趋势,‘特蕾1号’IAA含量先下降后上升,之后再下降再上升,‘大毛花’IAA含量先下降后上升。在大白期,‘特蕾1号’IAA、ZR含量为‘大毛花’的3.36倍和1.61倍,ABA、GA3含量为‘大毛花’的0.28倍和0.89倍;‘特蕾1号’IAA/ABA、ZR/ABA、IAA/GA3、ZR/GA3比值为‘大毛花’的11.96倍、5.67倍、3.80倍和1.81倍,ABA/GA3、ZR/IAA比值为‘大毛花’的0.32倍和0.45倍。影响‘特蕾1号’大白期时长的主要因素是高水平IAA和低水平ABA,还有高IAA/ABA、ZR/ABA、IAA/GA3及低ABA/GA3、ZR/IAA。本研究结果可供生产中利用外源激素调控忍冬花期,延长忍冬采收期,提高忍冬产量和质量,并为进一步研究花期延长的分子机理提供参考。

关键词: 忍冬, 花期时长, 内源激素, 延迟开花, 调控机理

Abstract: To study the regulation mechanism of plant endogenous hormones(IAA,ZR,GA3,ABA)on the flowering period of Lonicera japonica Thunb.,the main plant of the Lonicera japonica Thunb. cultivar‘Damaohua’flower and the new cultivar‘Telei 1’was used as experimental materials. The changes of flower color,morphology and flowering period in each developmental stage of two cultivars were observed,and the dynamics of four endogenous hormones in different developmental stages were detected by enzyme-linked immunosorbent assay. The results showed that the flowers of‘Telei 1’were larger than that of‘Damaohua’,and the two white period,great white period and falling period also lasted longer. During the whole flowering period,the contents of‘Telei 1’and ‘Damaohua’ZR and GA3 showed the trend of increasing and then decreasing,and the content of ABA showed a gradual increase trend. The content of IAA of‘Telei 1’firstly decreased,then increased,then falled and then rose again,the IAA content of the‘Damaohua’firstly decreased and then rose. In the great white period,the content of IAA and ZR in‘Telei 1’was 3.36 times and 1.61 times that of‘Damaohua’,and the content of ABA and GA3 was 0.28 times and 0.89 times that of‘Damaohua’. The ratios of IAA/ABA,ZR/ABA,IAA/GA3 and ZR/GA3 of‘Telei 1’were 11.96 times,5.67 times,3.80 times and 1.81 times of‘Damaohua’,and the ratios of ABA/GA3 and ZR/IAA were 0.32 times and 0.45 times that of‘Damaohua’. The main factors that affected the white period duration of‘Telei 1’were high IAA and low ABA content,as well as high ratio IAA/ABA, ZR/ABA,IAA/GA3 and low ratio ABA/GA3,ZR/IAA. In conclusion,with the use of exogenous hormones to control the flowering period,this study prolonged the harvest period of Lonicera japonica Thunb.,increased the yield and quality,and lay a foundation for further study of the molecular mechanism of flowering elongation.

Key words: Lonicera japonica, flowering prolongation, endogenous hormone, delayed flowering, regulation mechanism

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