羽衣甘蓝游离小孢子培养技术研究及应用

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 1019-1022.

羽衣甘蓝游离小孢子培养技术研究及应用

冯　辉^1*; 姜凤英^1,2; 冯建云¹; 王超楠¹

(¹ 沈阳农业大学园艺学院, 沈阳110161; ² 辽宁省农业科学院花卉研究所, 沈阳110161)

收稿日期:2007-01-15 修回日期:2007-05-08 出版日期:2007-08-25 发布日期:2007-08-25

Establishment and Application of the System for Isolated Microspore Culturein Kale ( Brassica oleracea L. var. acephala DC. )

FENG Hui^1*； JIANG Feng-ying^1,2； FENG Jian-yun¹；WANG Chao-nan¹

(¹College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; ² Floriculture Institute of Liaoning Academy of Agricultural Science, Shenyang 110161,China)

Received:2007-01-15 Revised:2007-05-08 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 以10个羽衣甘蓝杂交种为试材进行游离小孢子培养,研究胚状体发生和小孢子胚成苗的影响因素,以及小孢子植株倍性鉴定和单倍体加倍方法。结果表明,盛花期为最佳取样时期;培养基中13%的蔗糖较为适宜;继代培养以MS+6-BA 2 mg·L^-1+NAA 0.1 mg·L^-1为宜,平均增殖系数为5.06;小孢子再生植株成活率74.6%;小孢子植株自然加倍率为23.33%~37.50%;单倍体试管苗的加倍处理以秋水仙素浓度70 mg·L^-1,处理时间9~11d为宜,加倍率达54.55%。

关键词: 羽衣甘蓝, 小孢子培养, 加倍单倍体

Abstract: Isolated microspore culture was carried out by ten hybrids of kale (Brassica oleracea L. var. acephala DC.). The frequency of embryogenesis, embryos germinated plantlets, ploidy identification of microspores-derived plantlets and chromosome doubling techniques for haploid plantlets were studied. The results showed that the buds in flourishing florescence were the best for the purpose of microspore culture. The sucrose content of 13% was optimal in medium. MS+6-BA 2 mg·L-1+ NAA 0.1 mg·L^-1 was optimal medium for subculture and the average propagation quotiet reached 5.06. The survival rate of microspore-derived plantlets reached 74.6%. The DH plants rate of spontaneous was in the range of 23.33%-37.50%. The treatment of 70 mg·L^-1 colchicine for 9-11 d was optimal for haploid seedlings and led to 54.55% doubled haploid plantlets.

Key words: Kale, Brassica oleracea L. var. acephala DC., Microspore culture, Doubled hap loid

冯　辉;姜凤英;冯建云;王超楠. 羽衣甘蓝游离小孢子培养技术研究及应用[J]. 园艺学报, 2007, 34(4): 1019-1022.

FENG Hui;JIANG Feng-ying;FENG Jian-yun;WANG Chao-nan. Establishment and Application of the System for Isolated Microspore Culturein Kale ( Brassica oleracea L. var. acephala DC. )[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 1019-1022.

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