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园艺学报 ›› 2015, Vol. 42 ›› Issue (8): 1583-1590.doi: 10.16420/j.issn.0513-353x.2015-0218

• 研究报告 • 上一篇    下一篇

菠菜性别基因X/Y连锁标记的筛选及应用

刘丹丹,钱伟,张合龙,范桂彦,徐兆生   

  1. 中国农业科学院蔬菜花卉研究所,中蔬种业科技(北京)有限公司,北京 100081
  • 出版日期:2015-08-25 发布日期:2015-08-25
  • 基金资助:
    国家‘十二五’科技支撑计划项目(2011BAD35B07,2012BAD02B04,2014BAD01B08);国家自然科学基金项目(31401872);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业部园艺作物生物学与种质创制重点实验室项目

Development and Application of Molecular Markers Linked with Sex Gene X/Y in Spinach

LIU Dan-Dan, QIAN Wei, ZHANG He-Long, FAN Gui-Yan, XU Zhao-Sheng   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing Zhongshu Horticultural Crop Seed Research and Development Center,Beijing 100081,China
  • Online:2015-08-25 Published:2015-08-25

摘要: 以菠菜杂交一代品种‘冬绿1号’中的雌株与雄株杂交构建的F2分离群体为试验材料,采用SRAP-BSA法筛选与性别基因X/Y连锁的分子标记。从256对SRAP引物组合中筛选到了3个与性别基因X/Y紧密连锁SRAP标记:SRAP4.3、SRAP5.7和SRAP9.5。将SRAP5.7和SRAP9.5分别转化为SCAR标记(S5.7、S9.5),将SRAP4.3转化为dCAPs标记(D4.3)。SCAR标记S5.7、S9.5与Y基因共分离,dCAPs标记D4.3与X/Y基因紧密连锁,遗传距离为0.3 cM。利用S5.7、S9.5和D4.3在其它7个菠菜群体中进行雌雄性别鉴定,分子标记鉴定结果与表型鉴定结果一致,因此S5.7、S9.5和D4.3可以用于分子标记辅助选择育种。

关键词: 菠菜, 性别, SRAP, BSA法, SCAR, dCAPs, 分子标记辅助选择育种

Abstract: The F2 segregation population constructed by hybridization among female and male individuals of‘Winter Green 1’was our experimental material. We obtained the molecular markers linked with the sex gene X/Y using SRAP-BSA technique. Three SRAP markers(SRAP4.3,SRAP5.7,SRAP9.5)closely linked with the sex gene X/Y were gained from 256 SRAP primer combinations. SRAP5.7 and SRAP9.5 were successfully converted into SCAR markers(S5.7,S9.5). SRAP4.3 was smoothly transformed into dCAPs marker(D4.3). S5.7 and S9.5 marker were co-segregated with Y gene. D4.3 marker was closely linked to X/Y gene,and the genetic distance is 0.3 cM. The sex in other 7 spinach populations was identified by the marker S5.7,S9.5 and D4.3. The results of molecular identification were consistent with the phenotype,so these three molecular markers could be used for marker-assisted selection breeding(MAS).

Key words: spinach, sex, SRAP, BSA, SCAR, dCAPs, MAS

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