白菜类作物BrFLC5与开花时间相关的dCAPs标记开发

园艺学报 ›› 2014, Vol. 41 ›› Issue (10): 2035-2042.

白菜类作物BrFLC5与开花时间相关的dCAPs标记开发

张学铭，刘博，胡云艳，刘静，王晓武，武剑*

中国农业科学院蔬菜花卉研究所，北京100081

收稿日期:2014-05-19 出版日期:2014-10-25 发布日期:2014-10-25
基金资助:
国家重点基础研究发展计划（‘973’）项目（2012CB113900）；国家自然科学基金项目（31272179，30800753）

Development of dCAPs Marker for BrFLC5 Related with Flowering Time in Brassica rapa

ZHANG Xue-ming，LIU Bo，HU Yun-yan，LIU Jing，WANG Xiao-wu，and WU Jian*

Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Received:2014-05-19 Online:2014-10-25 Published:2014-10-25

摘要/Abstract

摘要： 为了揭示BrFLC5对白菜类作物抽薹开花的作用机制，克隆了12份开花习性不同的白菜类作物BrFLC5基因exon2 ~ exon4特异序列，分析发现在exon3的Pi3 + 1存在G–A变异，并开发了dCAPs标记。通过对来自10个不同栽培种群的93份材料进行开花时间调查和基因型检测，发现该基因的分子标记与开花时间表型显著相关（P < 0.05）。因此，用该标记可以进行抽薹开花分子标记辅助选择育种。

关键词: 白菜类, 抽薹开花时间, BrFLC5, dCAPs标记

Abstract: In order to reveal the mechanism of the BrFLC5 gene in the process of flowering time，we cloned exon2–exon4 of BrFLC5 in twelve Brassica rapa accessions with a wide range of flowering time variation. There was a single-base mutation（G–A）in the Pi3 + 1 of BrFLC5 exon3 and subsequently a dCAPs marker for BrFLC5 was developed in the B. rapa. We screened 93 accessions from ten different cultivar groups and analysed the correlation between flowering time and dCAPs marker for BrFLC5. The results showed that the flowering time was significantly correlated to the dCAPs of BrFLC5（P < 0.05）. The special marker can be easily used for marker-assisted selection（MAS）of the bolting and flowering time in breeding.

Key words: Brassica rapa, bolting and flowering time, BrFLC5, dCAPs marker

中图分类号:

S 634

张学铭, 刘博, 胡云艳, 刘静, 王晓武, 武剑. 白菜类作物BrFLC5与开花时间相关的dCAPs标记开发[J]. 园艺学报, 2014, 41(10): 2035-2042.

ZHANG Xue-Ming, LIU Bo, HU Yun-Yan, LIU Jing, WANG Xiao-Wu, WU Jian. Development of dCAPs Marker for BrFLC5 Related with Flowering Time in Brassica rapa[J]. ACTA HORTICULTURAE SINICA, 2014, 41(10): 2035-2042.